@article { author = {Tayoub, Ghaleb and Al-Odat, Mohmad and Amer, Amal and Aljapawe, Abdulmunim and Ekhtiar, Adnan}, title = {Antiproliferative Effects of Pancratium Maritimum Extracts on Normal and Cancerous Cells}, journal = {Iranian Journal of Medical Sciences}, volume = {43}, number = {1}, pages = {52-64}, year = {2018}, publisher = {Shiraz University of Medical Sciences}, issn = {0253-0716}, eissn = {1735-3688}, doi = {10.30476/ijms.2018.40513}, abstract = {Background: Plants are an important natural source of compounds used in cancer therapy. Pancratium maritimum contains potential anti-cancer agents such as alkaloids. In this study, we investigated the anti-proliferative effects of P. maritimum extracts on MDA-MB-231 human epithelial adenocarcinoma cell line and on normal lymphocytes in vitro. Methods: Leaves, flowers, roots, and bulbs of P. maritimum were collected and their contents were extracted and diluted to different concentrations that were applied on MDA-MB-231 cells and normal human lymphocytes in vitro for different intervals. Cells viability, proliferation, cell cycle distribution, apoptosis, and growth were evaluated by flow cytometry and microscopy. Parametric unpaired t-test was used to compare effects of plant extracts on treated cell cultures with untreated control cell cultures. IC50 was also calculated.Results: P. maritimum extract had profound effects on MDA-MB-321 cells. It inhibited cell proliferation in a dose- and time-dependent manner. The IC50 values were 0.039, 0.035, and 0.026 mg/ml after 48, 72, and 96 hours of treatment with 0.1 mg/ml concentration of bulb extract, respectively. Those values were 0.051 and 0.03 mg/ml after 72 and 96 hours for root extract, respectively, and 0.048 mg/ml after 96 hours for flower extract. There were no significant effects of P. maritimum bulb extracts on normal lymphocytes proliferation.Conclusion: P. maritimum extract has anti-proliferative effects on MDA-MB-231 cell line in vitro. The effects imply the involvement of mechanisms that inhibits cell growth and arresting cells at S and G2/M phases. Cyclin B1, Bcl-2, and Ki67 expression was also affected. }, keywords = {MDA-MB-231,Pancratium maritimum,Gene expression,Cell Proliferation,Lymphocytes}, url = {https://ijms.sums.ac.ir/article_40513.html}, eprint = {https://ijms.sums.ac.ir/article_40513_4c3d6101d7b64425cde052e4e60b347f.pdf} }