TY - JOUR ID - 39571 TI - Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Honarmand, Hamidreza AU - Falah Ghavidel, Mahsome AU - Nikokar, Iraj AU - Rahbar Taromsari, Morteza AD - Cellular and Molecular Research Center, Guilan University of Medical Sciences, Guilan, Iran AD - Department of Microbiology, Azad University, Lahijan, Iran AD - Department of Microbiology, School of Paramedicine, Guilan University of Medical Sciences, Guilan, Iran AD - Department of Legal Medicine, Razi Hospital, Guilan University of Medical Sciences, Guilan, Iran Y1 - 2012 PY - 2012 VL - 37 IS - 3 SP - 194 EP - 199 KW - Enterococcus faecalis KW - Multiplex-PCR KW - Van A KW - Van B DO - N2 - Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococcal bacteremia and its sensitivity pattern to antimicrobial drugs. Methods: Several bacterial suspensions with different content of two standard strains of Enterococcus faecalis resistant to vancomycin were used for inoculation to defibrinated sheep blood samples. PCR and routine assay was performed on all blood samples with different bacterial content. Results: Routine assay and PCR for all inoculated blood samples with ≥5 cfu/ml was positive. Mean time for PCR and routine assays was 10 hours and 5 days, respectively. Conclusion: PCR is a more rapid and sensitive assay for simultaneous detection and characterization for Enterococcus faecalis, and determination of its sensitivity pattern to vancomycin. UR - https://ijms.sums.ac.ir/article_39571.html L1 - https://ijms.sums.ac.ir/article_39571_091c4e84833824a355a0ac1356e55f68.pdf ER -