Document Type: Original Article(s)
Department of Medical Microbiology, Research Assistant in Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Anesthesiology, Medical Faculty, Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Microbiology, Boroujerd Branch, Islamic Azad University, Boroujerd, Iran
Department of Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
Department of Hematology, Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran; and Kurdistan Blood Transfusion Organization, Sanandaj, Iran
Department of Biology, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
Background: This study aimed to investigate the occurrence of aminoglycoside resistance and the prevalence of the resistance-modifying enzyme genes, ant(3”)-III, ant(6’)-Ia, aac(6′)-Ie-aph(2”)-Ia, and aph(2’)-Id, in Enterococcus strains isolated in Kermanshah Province, west of Iran.Methods: In this cross-sectional study, 108 enterococcal isolates from urine, wound, blood, and cerebrospinal fluid samples were collected. The Enterococcus species were recognized by standard phenotypic/biochemical tests. The antimicrobial resistance forms were detected using a disc diffusion method. Polymerase chain reaction was designed to identify aminoglycoside resistance genes, including ant(3”)-III, ant(6’)-Ia, aac(6′)-Ie-aph(2”)-Ia, and aph(2’)-Id.Results: Totally, 108 strains with a final diagnosis of Enterococcus were extracted from 84 (77.8%) urine, 14 (13%) wound, 6 (5.6%) blood, and 4 (3.7%) cerebrospinal fluid samples. Among the 108 Enterococcus specimens, 94 (87%) cases were Enterococcus faecalis and 14 (13%) were Enterococcus faecium. The highest frequency of resistance was observed for erythromycin (88.9%), while the lowest was found for streptomycin (44.4%). The frequency of high-level gentamicin resistance was 42.2%. Among the identified specimens, 42.6% contained the aac(6′)-Ie-aph(2”)-I gene, 20.4% contained the ant(6’)-Ia gene, and 15.7% contained the ant(3”)-III gene. A significant correlation was found between phenotypic gentamicin resistance and the presence of the aminoglycoside resistance genes (P<0.05). Conclusion: This study showed the high resistance of Enterococcus strains isolated from hospital samples. Compared with the previous studies, the strains isolated in our study showed a higher percentage of resistance to aminoglycosides.