Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

Effect of Resveratrol Supplementation on the SNARE Proteins Expression in Adipose Tissue of Stroptozotocin-Nicotinamide Induced Type 2 Diabetic Rats

Document Type : Original Article(s)

Authors

Azam Rezaei Farimani 1
Massoud Saidijam 2
Mohammad Taghi Goodarzi 2
Reza Yadegar azari 3
Soheila Asadi 1
Sadegh Zarei 1
Nooshin Shabab 3

1 Department of Biochemistry, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

2 Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan Iran

3 Department of Genetic and Molecular Medicine, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran

Abstract
Background: Glucose uptake by muscles and fat cells is carried out by the GLUT4 system. Isoforms of the SNAP23, syntaxin-4 and VAMP-2 play an important role in regulating GLUT-4 trafficking and fusion in adipocytes. The changes of SNARE proteins levels and thus impaired GLUT-4 displacement can be one of the etiological causes of type 2 diabetes.Due to changes in the expression of these proteins in diabetes, the aim of this study was to investigate the effect of the natural compound resveratrol with anti-diabetic properties on impaired expression of SNARE proteins in type 2 diabetes.Methods: Forty male Wistar rats were used in this study. Type 2 diabetes was induced by administering a single dose of streptozotocin and nicotinamide. The expression of SNAP-23, syntaxin-4 and VAMP-2 proteins were assessed using real-time qRT-PCR. Also, some biochemical parameters were examined, including fasting blood glucose, insulin levels and insulin resistance.Results: The results of this study showed that, resveratrol supplementation increased blood insulin level, reduced the fasting blood glucose, and improved the insulin resistance. In addition, resveratrol supplementation increased the expression of SNAP-23, syntaxin-4 and VAMP-2 proteins that involved in GLUT-4 transport in adipose tissue of diabetic rats.Conclusion: Final results showed that SNARE proteins expression is significantly reduced in diabetic rats and treatment with resveratrol supplementation is associated with the increased expression of these proteins.

Keywords

GLUT4
SNAP23 Protein
Syntaxin-4 Protein
Vesicle-associated membrane Protein 2
Resveratro

  • Received 11 April 2014
  • Revised 22 August 2020
  • Accepted 24 December 2018

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