TY - JOUR ID - 44955 TI - Negative Regulation of Semaphorin-3A Expression in Peripheral Blood Mononuclear Cells Using MicroRNA-497-5p JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Shapoori, Shima AU - Ganjalikhani-Hakemi, Mazdak AU - Rezaeepoor, Mahsa AU - Alsahebfosoul, Fereshteh AU - Khosravi, Sharifeh AU - Etemadifar, Masoud AU - Mansourian, Marjan AD - Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran AD - Department of Genetics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran AD - Multiple Sclerosis and Neuroimmunology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran AD - Department of Biostatistics and Epidemiology, Faculty of Health, Isfahan University of Medical Sciences, Isfahan, Iran Y1 - 2019 PY - 2019 VL - 44 IS - 4 SP - 325 EP - 333 KW - autoimmune disease KW - MicroRNAs KW - Mononuclear phagocyte system KW - Semaphorin-3A DO - 10.30476/ijms.2019.44955 N2 - Background: Semaphorin-3A (Sema3A), as a secreted semaphorin, is an immune modulator molecule participating in the pathogenesis of autoimmune diseases. MicroRNAs (miRNAs) modulate the target-gene expression at the post-transcriptional level. It has been proposed that miRNAs may be crucial to the modulation of the function of semaphorins. Previous findings have proven that miR-497-5p is upregulated and Sema3A is downregulated in some autoimmune disorders. Thus, we aimed to examine the presence of any correlation between Sema3A and miR-497-5p in peripheral blood mononuclear cells (PBMCs). Methods: PBMCs were cultured and transfected with miR-497-5p mimic using the X-tremeGENEā„¢ reagent. The expression level of Sema3A was assessed after 48 hours in supernatants and cells via the enzyme-linked immunosorbent assay and quantitative real-time polymerase chain reaction, respectively. Cell viability was evaluated using the methylthiazol tetrazolium assay. All the experiments were done in triplicate, and the statistical analyses were performed with SPSS, version 20. P values equal to or less than 0.05 were considered significant.Results: We observed downregulation of the Sema3A gene (P=0.0001) and its secretion (P=0.032) in the PBMCs through miR-497-5p transfection. Moreover, transfection with miR-497-5p mimic and downregulation of Sema3A did not affect the viability of the PBMCs (P=0.061).Conclusion: Based on the obtained results, we suggest that miR-497-5p has a high suppressive effect on Sema3A expression and both Sema3A and miR-497-5p can be considered critical targets for further studies on future therapeutic attempts for the treatment of autoimmune diseases such as multiple sclerosis and rheumatoid arthritis. UR - https://ijms.sums.ac.ir/article_44955.html L1 - https://ijms.sums.ac.ir/article_44955_601b26541cf1caf899f855ce6d2e051c.pdf ER -