TY - JOUR ID - 44971 TI - The Hydroalcoholic Extract of Saffron Protects PC12 Cells against Aluminum-Induced Cell Death and Oxidative Stress in Vitro JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Rahmani, Shokofeh AU - Saberzadeh, Jamileh AU - Takhshid, Mohammad Ali AD - School of pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran AD - Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran AD - Diagnostic Laboratory Sciences and Technology Research Center, School of Paramedical Sciences, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2020 PY - 2020 VL - 45 IS - 1 SP - 59 EP - 66 KW - Aluminum maltolate KW - Crocus KW - PC12 cells KW - apoptosis KW - Oxidative stress DO - 10.30476/ijms.2019.44971 N2 - Background: Aluminum (Al) exposure is among the environmental risk factors that may involve in the pathogenesis of neurodegenerative diseases. Oxidative stress has a critical role in the Al-induced toxicity. Saffron is a plant with potent radical scavenging and anti-oxidative properties. This investigation was designed to evaluate the possible protective effects of saffron extract (SE) on aluminum maltolate (Almal)-induced oxidative stress and apoptosis in PC12 cell line. Methods: In this in vitro study, PC12 cells were divided into four groups including control, Almal (500 µM), Almal+SE (50 μg/ml), and Almal+SE (100 μg/ml). After 48 hours of treatment with Almal in the absence and presence of SE, cell viability and apoptosis were determined using MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay and Annexin V flow cytometry, respectively. Catalase activity was determined as an index of oxidative stress. Statistical analyses were performed using one-way ANOVA (SPSS version 16.0). PResults: Almal decreased the PC12 cells viability dose-dependently (IC50=500µM). Co-treatment of 50 and 100 μg/ml of SE with 500 µM of Al increased cell viability to 79% (P=0.04) and 86% (P=0.02) of the control group, respectively. Al also increased PC12 cells apoptosis and catalase activity to 37 and 2.7 folds of those of the control group (PConclusion: SE has protective effects against Al-induced apoptosis and oxidative stress and may possess therapeutic values in the treatment of Al-neurotoxicity. UR - https://ijms.sums.ac.ir/article_44971.html L1 - https://ijms.sums.ac.ir/article_44971_dca6bf11a2b458c0166e4b90f0f4bea1.pdf ER -