TY - JOUR ID - 40568 TI - Safety Assessment of Mentha mozaffarianii Essential Oil: Acute and Repeated Toxicity Studies JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Daneshbakhsh, Dorsa AU - Asgarpanah, Jinous AU - Najafizadeh, Parvaneh AU - Rastegar, Tayebeh AU - Mousavi, Zahra AD - Herbal Medicines Research Center, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran AD - Department of Pharmacognosy, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran; and Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran AD - Department of Pharmacology, Iran University of Medical Sciences, Tehran, Iran; and Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran AD - Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran AD - Department of Pharmacology and Toxicology, Faculty of Pharmacy, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran Y1 - 2018 PY - 2018 VL - 43 IS - 5 SP - 479 EP - 486 KW - Mentha KW - Mentha mozaffarianii KW - Essential oil KW - Toxicity KW - Rat KW - mice DO - 10.30476/ijms.2018.40568 N2 - Background: Mentha mozaffarianii, an endemic species from the Labiatae family, is used in Iranian traditional medicine. This study evaluated the acute and repeated oral toxicity of the Mentha mozaffarianii essential oil (MMEO) in rats and mice. Methods: To assess the toxicity profile of the MMEO, we administered the essential oil to 48 rats and mice of both sexes by gavage in acute and repeated models. In acute toxicity, the animals were administered the MMEO (2000 mg/kg) and were monitored for 14 days. In the repeated toxicity, the MMEO was administered (100 mg/kg) daily for 4 weeks. On the 28th day, all the animals were scarified and blood and tissue samples were prepared. All the clinical, biochemical, and histopathological changes were assessed and compared with those in the controls. Statistical significance was determined by one- and two-way analyses of variance, followed by the Tukey test using GraphPad Prism 6. Results: In the acute test, there was no mortality; therefore, the oral LD50 value determined in the mice and rats of both sexes was greater than 2000 mg/kg. In the repeated test, the animals received the MMEO and there was no mortality. In the biochemical analysis, there were significant increases in blood glucose, cholesterol, ALT, AST, ALP, and TSH in the female rats and also in BUN in the male rats. The histopathological studies revealed evidence of microscopic lesions in the liver, kidney, stomach, and small intestine tissues of the MMEO group. Conclusion: The results indicated that the acute toxicity of the MMEO in the mice and rats was of a low order and it revealed slight tissue damage to several organs when given subchronically at a dose of 100 mg/kg. UR - https://ijms.sums.ac.ir/article_40568.html L1 - https://ijms.sums.ac.ir/article_40568_bb86fbce4b114444589966510bdbc0df.pdf ER -