TY - JOUR ID - 45420 TI - Characterization and Lytic Activity of Isolated Escherichia Coli Bacteriophages against Escherichia Coli in Vitro JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Rahimzadeh, Golnar AU - Resch, Gregory AU - Rezai, Mohammad Sadegh AU - Nemati Hevelaee, Ebrahim AD - Pediatric Infectious Diseases Research Center, Mazandaran University of Medical Sciences, Sari, Iran AD - Department of Fundamental Microbiology, University of Lausanne, Lausanne, Switzerland AD - Laboratory of microbiology, Bouali Sina Hospital, Mazandaran University of Medical Sciences, Sari, Iran Y1 - 2020 PY - 2020 VL - 45 IS - 4 SP - 298 EP - 303 KW - Escherichia coli KW - Bacteriophages KW - Urinary tract infections DO - 10.30476/ijms.2019.45420 N2 - Background: Escherichia coli (E. coli) is the most common cause of urinary tract infection (UTI) and typically treated with antibiotics. Unrestricted use of antibiotics may lead to the emergence of antibiotic-resistant bacteria. The present study aimed to isolate and characterize phages against E. coli from infected urine samples and to determine the lytic activity of phages against E. coli in vitro.Methods: The present experimental study was conducted in the Laboratory of Bouali Sina Hospital (Sari, Iran) in May 2018. E. coli was identified from nine urine samples of patients with UTI using conventional microbiological methods. Bacteriophages were isolated from the infected urine specimens, and their lytic activity was determined using the spot test. The titer of the bacteriophages was measured using the double-layer agar technique. The morphology of the bacteriophages was revealed using transmission electron microscopy, and the latent time period and burst size were determined. Data were analyzed using the SPSS software package.Results: E. coli was isolated from nine infected urine samples. The lytic activity of bacteriophages against E. coli was determined using the spot test by observing the formation of inhibition zones. Transmission electron microscopy showed E. coli phages belonging to the Myoviridae family. The latent time period was 20 minutes with a burst size of 1,200 plaque-forming unit (PFU) per infected cell. The results of the double-layer agar assay showed that the titer of bacteriophages was 20×108 PFU/mL.Conclusion: The E. coli bacteriophage was isolated from infected urine samples and characterized, and their lytic activity against E. coli was determined in vitro. UR - https://ijms.sums.ac.ir/article_45420.html L1 - https://ijms.sums.ac.ir/article_45420_a7a0d040a0cc667dc99548e29f2523d3.pdf ER -