TY - JOUR ID - 40587 TI - Evaluation of the Possible Synergic Regenerative Effects of Platelet-Rich Plasma and Hydroxyapatite/Zirconia in the Rabbit Mandible Defect Model JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Shahsavari-Pour, Sheila AU - Aliabadi, Ehsan AU - Latifi, Mona AU - Zareifard, Nehle AU - Namavar, Mohammad Reza AU - Talaei-Khozani, Tahereh AD - Department of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz Iran AD - Department of Tissue Engineering, National Institute of Genetic Engineering and Biotechnology, Iran AD - Stem Cell Lab, Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AD - Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran AD - Tissue Engineering Lab, Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran Y1 - 2018 PY - 2018 VL - 43 IS - 6 SP - 633 EP - 644 KW - Durapatite KW - Hydroxyapatite KW - Zirconium oxide KW - Platelet-Rich plasma KW - Heparan sulfate proteoglycans KW - Osteogenesis DO - 10.30476/ijms.2018.40587 N2 - Background: Platelet-rich plasma (PRP) and bioceramics such as hydroxyapatite (HA) and zirconium oxide (ZrO2) are used to reconstruct mandibular defects. We sought to determine the synergistic effects of HA/ZrO2 and PRP and compare their osteogenic activity.Methods: ZrO2 scaffolds were constructed by the slurry method and were then coated with HA and impregnated by PRP/heparan sulfate (HS). Bilateral mandibular defects were created in 26 male rabbits. In 20 rabbits, the left defects were treated with HA/ZrO2/PRP (Group 1) and the corresponding right defects were filled with HA/ZrO2 (Group 2). The 6 remaining models were treated with PRP gels at both sides (Group 3). The osteoconductivity of HA/ZrO2/PRP was compared with that of HA/ZrO2 or PRP by radiological and histological methods after the follow-up period, at weeks 6, 8, and 12. The statistical analyses were performed by ANOVA and LSD using SPSS, version 16.0, for Windows (P<0.05).Results: After 2 weeks, the percentage of the surface occupied by bone was significantly higher in the HA/ZrO2/PRP-treated defects than in the PRP-treated defects (P=0.007). Osteoblast and osteocyte counts were higher significantly in the PRP-treated group (P=0.032); however, the cells had not started matrix formation on a large scale and just small islands of osteoid with trapped osteocytes were observed. In the long term, the regenerative potential of all the scaffolds was the same. Conclusion: HA/ZrO2 showed a superior osteoconductive capacity over PRP in the short term; however, they showed no long-term synergic effects. UR - https://ijms.sums.ac.ir/article_40587.html L1 - https://ijms.sums.ac.ir/article_40587_9caecb0759309c74865227b432b55a84.pdf ER -