TY - JOUR ID - 39647 TI - The Expression of Heme Oxygenase-1 in Human-Derived Cancer Cell Lines JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Habibi Roudkenar, Mehryar AU - Bahmani, Parisa AU - Hassanshahi, Golamhossein AU - Halabian, Raheleh AU - Mohammadi Roushandeh, Amaneh AU - Jahanian-Najafabadi, Ali AD - Research Center, Iranian Blood Transfusion Organization, Tehran, Iran AD - Molecular Medicine Research Center, Rafsanjan University of Medical Sci-ences, Rafsanjan, Iran AD - Department of Anatomy, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran AD - Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran Y1 - 2011 PY - 2011 VL - 36 IS - 4 SP - 260 EP - 265 KW - Heme oxygenase-1 KW - Gene expression KW - HepG2 KW - A549 KW - MCF7 KW - K562 KW - cancer cells DO - N2 - Background: Heme oxygenase-1 (HO-1) is a cytoprotective and antiapoptotic enzyme, which has been involved in maintaining cellular homeostasis, and plays an important protective role by modulating oxidative injury. Up-regulation of (HO-1) has contributed to tumorogenicity of some cancers. In this study we investigated the expression pattern of the HO-1, in five different human-derived cancer cell lines with high incidence in Iran. Methods: Total cell RNA were extracted from HepG2 (hepato carcinoma), A549 (lung adenocarcinoma), MCF-7 (breast cancer), K562 (myeloid leukemia) and LS174T (colon cancer) cell lines. Human embryonic kidney (HEK293) cell line was used as a control. cDNAs were synthesized and expression of HO-1 was examined using RT-PCR. Results: The expression of HO-1 was not detected in the control cell line (HEK293), but it was observed to express following ultraviolet (UV) exposure indicating that HO-1 is not constantly expressed. The examined cancer cell lines constitutively expressed different variety of HO-1 on mRNA level. Strong expression of HO-1 was observed in HepG2, MCF-7 and A549 cells. A moderate expression of HO-1 was observed in K562 cells, and LS174T cells showed no expression of HO-1. Conclusion: Heme oxygenase-1 could be considered as a new marker in the diagnosis of some cancers, especially hepatomacarcinoma. Our results also suggest that up-regulation of HO-1 may contribute to tumorogenicity of some cancers. Therefore, the combination of gene-silencing effect of HO-1 and chemotherapy might be considered as a new modality for the treatment of cancers in which the expression HO-1 is up-regulated. UR - https://ijms.sums.ac.ir/article_39647.html L1 - https://ijms.sums.ac.ir/article_39647_de250f707e9d66fbee53a14c0d3d4863.pdf ER -