eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
51
55
40127
Original Article(s)
Langerhans Cells in Skin Lesions of Leprosy
B. Azadeh
bazadeh@liv.ac.uk
1
S. Dabiri
2
Departments of Pathology,
The Liverpool University,
Liverpool, UK and
Departments of Pathology,
Kerman University of Medical Sciences, Kerman, Iran
Background: Langerhans cells are important in the pathogenesis of leprosy. A recent study reported that these cells were almost absent in the epidermis overlying lepromatous lesions. Objective: To investigate a possible relationship between the number of Langerhans cells and the histopathologic spectrum of lesions in patients with leprosy. Methods: An immunohistochemical staining for S100 protein was used to study skin punch biopsies of patients with leprosy and to quantify S100-positive Langerhans cells in the epidermis. Results: A progressively significant reduction in the number of Langerhans cells was found from tuberculoid to lepromatous skin lesions. Langerhans cells were significantly less frequent in lepromatous and borderline lepromatous as compared to either tuberculoid or borderline tuberculoid leprosy. Conclusion: In view of the fact that adequate numbers of dendritic cells are recruited to the dermis, their maturation/migration to Langerhans cells in the epidermis is impaired in lepromatous leprosy.
https://ijms.sums.ac.ir/article_40127_acbdf639ad480303c71096cce3159689.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
56
61
40130
Original Article(s)
E-Cadherin in Relation with the Proliferating Cell Nuclear Antigen of the Bilharzia Associ-ated and Non-Associated Urinary Bladder Carcinoma
M.M. Rashed
drmonarashed@hotmail.com
1
N.M. Rageb
2
General Organization for Teach-ing Hospitals and Institutes,
Cairo Medical Research Institute, Alexandria University,
Alexandria, Egypt
General Organization for Teach-ing Hospitals and Institutes,
Cairo Medical Research Institute, Alexandria University,
Alexandria, Egypt
Background: E-cadherin is a trans-membrane glycoprotein that plays a critical role in many aspects of cell adhesion as well as establishment and maintenance of epithelial cell polarity. Loss of the adhesive function of E-cadherin seems to promote invasive and metastatic properties of neoplastic cells. Objectives: The present study is a retrospective study aiming to evaluate the loss of E-cadherin immunohistochemical expression in relation with the proliferating cell nuclear antigen expression of the bilharzia associated and non-associated bladder carcinoma. Methods: Forty TUR-bladder carcinoma sections immunohistochemicaly stained with E-cadherin antibody were microscopically interpreted and results were correlated to the established prognostic factors, including proliferating index as assessed by the proliferating cell nuclear antigen (PCNA) immunostaining, histopathological types, tumor cell grade, tumor invasiveness and bilharzia association. Results: Histopathologically, 63% were transitional cell carcinoma, 33% were squamous cell carcinoma and 5% were adenocarcinoma. Loss of E-cadherin expression had a significant association with high PCNA index (p<0.01), the tumor grade (p<0.002), tumor invasiveness (p<0.001), and bilharzias-associated bladder cancer (p<0.04). There was no statistically significant association between loss or decrease of E-cadherin expression and histopathological typing of urinary bladder carcinoma (p=0.094). Conclusion: Loss of E-cadherin provides an additional aid in assessment of prognosis and planning of therapy of patients with urinary bladder carcinoma as it indicates the potentiality for metastasis by its significant association with high proliferating cell nuclear antigen index, high tumor grade and tumor invasiveness. Moreover, immunohistochemical interpretation of E-cadherin altered adhesive function is a useful histological prognostic marker in bilharzia associated urinary bladder carcinoma.
https://ijms.sums.ac.ir/article_40130_ad33cea8a63b31bf47d78bf1d42569b8.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
62
66
40131
Original Article(s)
Emotional Changes in Children Undergoing Cochlear Implantation through Evaluation of Their Drawings
S. Abdi
s.abdi_aalam@kosar.net
1
B. Jalili
2
H. Tavakoli
hearingcentertums@neda.net
3
M. Naderpour
4
Cochlear Implantation Clinic,
Hearing Research Center,
Amir Aalam Hospital,
Tehran University of Medical Sci-ences,
Tehran, Iran.
Iran University of Medical Sciences, Esmaili Child Psychiatry Center,
Tehran, Iran.
Departmant of General Surgery, Tehran University of Medical
Sciences,
Tehran, Iran.
Department of ENT, Tabriz University of Medical Sciences, Tabriz, Iran.
Background: The emotional changes of patients undergoing cochlear implantation (CI) has been addressed in a few studies. To date, no special test has been developed to measure these changes. Objective: To measure the emotional status of children undergoing CI by interpreting their drawings. Methods: A prospective longitudinal study on prelingually deafened children from 4-14 years of age undergoing CI and sex and age-matched non-CI candidate deaf children comprised the study population. Drawings were produced before implantation and after 1, 3, 6, and 12-month intervals. These were scored with regard to a questionnaire developed inAmirAalamHospitalHearingResearchCenter,Tehran,Iran. Results: The prevalence of drawings with cues of rage/anxiety decreased from 35% to 21.9%. Similarly, sadness prevalence dropped from 36.8% to 20% and the prevalence of immaturity was reduced from 20% to 7.1%. Conclusion: We recommend drawing to be included in the rehabilitation program of children undergoing CI and used as a practical measure of their emotional progress.
https://ijms.sums.ac.ir/article_40131_3b0ffe5df6014d1434f375715b5b9814.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
67
71
40132
Original Article(s)
Polymorphisms in the Apolipoprotein E Region and Severity of Multiple Sclerosis
V. Hadavi
hvaleh2000@yahoo.com
1
D.D. Farhud
2
M.H. Sanati
3
S.M. Nabavi
4
M. Seyedian
5
M. Hushmand
6
M. Younesian
7
Dept. of Human Genetics & Anthropology, School of Public Health & Institute of Public Health Research, Tehran University of Medi-cal Sciences,
Tehran, Iran
National Research Center for Genetic Engineering and Biotechnology,
Tehran University of Medical Sciences,
Tehran, Iran.
National Research Center for Genetic Engineering and Biotechnology,
Tehran University of Medical Sciences,
Tehran, Iran.
Shahed Medical University, Depart-ment of Neurology,
Tehran University of Medical Sciences,
Tehran, Iran.
Roozbeh Hospital,
Tehran University of Medical Sciences,
Tehran, Iran.
National Research Center for Genetic Engineering and Biotechnology,
Tehran University of Medical Sciences,
Tehran, Iran.
Dept. of Environmental Health Engineering , School of Public Health & Institute of Public Health Research,
Tehran University of Medical Sciences,
Tehran, Iran.
Background: The apolipoprotein E (APOE) polymorphism is known to affect various neurologic disorders with different effects on the immune system and CNS repair. However, previous studies on possible modulation of the clinical course of multiple sclerosis (MS) by APOE polymorphism have been inconsistent. Objective: To clarify the issue for MS patients' management and future research. Methods: The present cross-sectional study investigated 81 patients with clinically proven MS and related their clinical and demographic findings to the allelic polymorphism of the APOE gene. The genotype distribution of patients with MS was compared with a comparison group of 93 asymptomatic elderly volunteers. Results: Significant differences were found in the distribution of e4 allele between patients with MS and the comparison group (9.3% vs. 0.5%; p<0.001). An analysis of disease progression in 81 patients with MS indicated that APOE e4 carriers are more likely to be affected with severe disease. Conclusion: The results obtained suggested that APOE genotype affected susceptibility to MS and indicated an association of the APOE e4 allele with a more severe course of the disease.
https://ijms.sums.ac.ir/article_40132_8d5f8cc6ffa96a2971c8784bb57bdfb6.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
72
74
40133
Original Article(s)
High Intravenous Fluid Therapy Prevents Post-Tonsillectomy Nausea and Vomiting
F. Heshmati
nader5372001@yahoo.com
1
A. Hasani Afshar
2
A. Mahoori
3
M.B. Zeinali
4
R. Abbasivash
5
Departments of anesthesiology and
Urmia University of Medical Sciences
Urmia, Iran.
ENT Emam Khomeini Hospital,
Urmia University of Medical Sciences
Urmia, Iran.
Departments of anesthesiology and
Urmia University of Medical Sciences
Urmia, Iran.
Departments of anesthesiology and
Urmia University of Medical Sciences
Urmia, Iran.
Departments of anesthesiology and
Urmia University of Medical Sciences
Urmia, Iran.
Background: Post-operative nausea and vomiting (PONV) following surgical operations requiring general anesthesia are common and distressing. The incidence of PONV may be as high as 70% during the first 24 hrs of tonsillectomy. Objective: This study determines the effects of intraoperative well-hydration on postoperative nausea and vomiting. Methods: 90 ASA I patients with age of 6-12 years scheduled for tonsillectomy under general anesthesia randomly assigned to receive either routine iv fluid plus intraoperative well-hydration plus 4 ml/kg/h Ringer’s solution (well hydrated group; n=45) and routine iv fluid requirements (control group; n=45). All study preparations were administered in a double-blinded fashion. Results: During the first postoperative day, the incidence of nausea and vomiting were significantly lower in the well-hydrated group as compared with control (p<0.05). There was no significant differences between males and females regarding the incidence of nausea and vomiting (p>0.05). Conclusion: The present study showed that well-hydration reduces the incidence of post tonsillectomy nausea and vomiting, and high iv fluid therapy is a simple, effective, safe and well-tolerated technique for prevention of postoperative nausea and vomiting.
https://ijms.sums.ac.ir/article_40133_8ca62f120a54876abf8f6eaa676ff1c4.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
75
80
40134
Original Article(s)
Nephrotoxicity of Isosorbide Dinitrate and Cholestasis in Rat: The Possible Role of Nitric Oxide
A.M. Sharifi
sharifal@yahoo.com
1
N. Akbarloo
2
F. Sasani
3
Department of Pharmacology,
School of Medicine, Endocrinology and Metabolism Research Center (EMRC),
Dr Shariati Hospital, Tehran University of Medical Sciences,
Tehran, Iran
Department of Pharmacology,
School of Medicine, Endocrinology and Metabolism Research Center (EMRC),
Dr Shariati Hospital, Tehran University of Medical Sciences,
Tehran, Iran
Department of Pathology,
Veterinary School, Tehran University,
Tehran, Iran
Background: Nitric oxide (NO), a major chemical form of endothelium-derived relaxing factor and an important regulator of vascular tone, is released by endothelial cells. The role of NO is not restricted to the vascular system, and it participates in the regulation of renal hemodynamics and renal excretory function. There are increasing evidences indicating that the elevated levels of NO play a primary pathogenic role in the glomerular injury and renal failure. Objectives: We sought to investigate the renoprotective or nephrotoxic effects of various doses of isosorbide dinitrate as an exogenous model, and induction of cholestasis as an endogenous model of NO overproduction on renal function and structure. Methods: Parameters such as plasma and urine p-nitrophenyl-N-acetyl-β-D-glucosaminidase (NAG)-activity, urea and creatinine levels were measured. Results: Urea, creatinine and NAG-activity in rats treated by different doses of isosorbide as well as in cholestasis induced rats, were higher than control group. This elevation was significantly pronounced at higher doses of isosorbide. Conclusion: NO overproduction would be nephrotoxic due to oxidizing products of NO, peroxynitrite anion (ONOO–), formed by the reaction of NO and superoxide radical (O2–).
https://ijms.sums.ac.ir/article_40134_dd4a8b4775256a014d0143984aa82f57.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
81
84
40135
Original Article(s)
Survival and Chemotactic Behavior of H pylori at Different Media pH
O. Tadjrobehkar
tadjrobehkar@yahoo.com
1
H. Abdollahi
2
Zabol University of Medical Sciences, Zabol, Iran
Microbiology Dept, Medical school, Kerman University of Medical Sciences, Kerman, Iran
Background: H pylori is a human gastric pathogen. Chemotaxis is an essential factor in colonization of H pylori, but very little is known about its chemotactic responses at different pH conditions, especially in acidic environment of stomach as its natural habitant. Methods: We first determined survival of H pylori under various pH conditions in the presence and absence of urea. Chemotaxis was then assayed in three strains of H pylori by modified Adler’s procedure, in which potassium bicarbonate was used as an attractant for estimating the chemotactic activity which was compared with phosphate buffer as an inert reagent. Results: H pylori cannot withstand pH 1, but in the presence of urea, it survives in solutions with pH values of2 to 9and remains viable at pH of 3 to 9, irrespective of presence of urea. Maximum chemotactic activity occurred at pH values of 5.5 to 6.5, whereas no chemotaxis was found in solutions with pH 3. Chemotactic activities are increased at pH 3 to 5.5 and reduced at pH 6 to 9, beyond which no chemotaxis was observed. Conclusion: In view of the fact that chemotaxis is severely affected by media pH, it is concluded that the antrum of the stomach is most suitable for colonization of H pylori for which maximum colonization occurs at neutral pH.
https://ijms.sums.ac.ir/article_40135_1a127d448dd4101482d64856d8724280.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
85
89
40136
Original Article(s)
Doppler-Derived Myocardial Performance Index in Healthy Children in Shiraz
M. Borzoee
borzoeem@sums.ac.ir
1
Z. Kheirandish
2
Pediatric Cardiology Division,
Pediatrics Department,
Shiraz University of Medical Sci-ences, Shiraz, Iran
Pediatric Cardiology Division,
Pediatrics Department,
Shiraz University of Medical Sci-ences, Shiraz, Iran
Background: Assessment of myocardial function is essential in heart disease, but in regard to systolic and diastolic functions such evaluation has limitation. Ejection fraction is difficult to assess in abnormally-shaped ventricles, and diastolic inflow velocity pattern may be fused because of tachycardia. Objective: A myocardial performance index (MPI) or Tei index has been developed for adults and children. It is a Doppler derived non-geometric measure of ventricular function and is independent of heart rate. This index measures the ratio of isovolumic contraction time plus isovolumic relaxation time to ventricular ejection time. Methods: We studied 108 healthy children from 3 days to18 years of age, in whom ejection fraction was measured using M-mode (73±8%) and 2-dimensional echocardiogram biplane Simpson's method (62±7%). Results: Right and left ventricular myocardial performance indices were 0.25 ±0.09 and 0.36 ±0.11 respectively. No correlation was found between Echo Doppler index and age, heart rate and left ventricular dimensions. Conclusion: Thus, MPI is a simple and accurate tool for quantitative assessment of right and left ventricular functions and because of easy application and reproducibility; it could be regarded as an important measurement in a comprehensive hemodynamic study, especially in those with abnormal ventricular geometry.
https://ijms.sums.ac.ir/article_40136_1f94497ba17f34d150ae23871a5871a3.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
90
93
40137
Original Article(s)
Depression among Parents of Children with Chronic and Disabling Disease
H. Ashkani
psychiat@sums.ac.ir
1
Gh. R. Dehbozorgi
2
A. Tahamtan
3
Department of Psychiatry,
Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Psychiatry,
Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Psychiatry,
Shiraz University of Medical Sciences, Shiraz, Iran.
Background: The parent’s awareness about chronic disabling disease in their children definitely distresses the parents. Children of such parents show behavior & cognitive disturbances with long term negative impact on the family. Objective: The present study was carried out to investigate the prevalence of depression in parents of disabled children. Methods: Following a cross-sectional study, 50 parents of children with chronic disease as target group & 50 parents of children with an acute disease as control group were selected randomly, they were asked to take part in a structured interview & their depression’s score was measured using the Beck Depression Inventory (BDI). Data analysis was performed by Student’s t test and ANOVA using SPSS software. Results: Depression scores were higher in those who had a child with chronic disease than in the control group (P<0.001). Depression scores were also higher in those who had a leukemic child than other non-leukemic chronic disease (P<0.002). Lower socioeconomic (SES) & parents with lower education level also showed a higher level of depression. Conclusion: When comparing psychopathology in parents of children with chronic disease with that of children with an acute disease, significant differences were observed in depression level. This study emphasizes the necessity of active psychosocial intervention in those parents who have disabled children.
https://ijms.sums.ac.ir/article_40137_74beded8ca59cc9c4feb978589dc5608.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
94
96
40128
Brief Report(s)
Bone Scintigraphy in Acute Lymphoblastic Leukemia
M. Alavi
1
A. Nabavizadeh
2
A. Mowla
3
M. Karimi
4
Departments of Nuclear Medicine
Shiraz University of Medical Sciences, Shiraz, Iran
Hemostasis and Thrombosis unit, Hematology Research center,
Shiraz University of Medical Sciences, Shiraz, Iran
Hemostasis and Thrombosis unit, Hematology Research center,
Shiraz University of Medical Sciences, Shiraz, Iran
Hemostasis and Thrombosis unit, Hematology Research center,
Shiraz University of Medical Sciences, Shiraz, Iran
Leukemia is the most common childhood cancer and accounts for 30-40% of all malignancies. A retrospective review was performed of the hospital records of 9 children, 6 boys and 3 girls, aged from 2.5 to 15 years with ALL initially referred to Nemazee hospital Nuclear medicine center for whole body bone scanning between 2000 and 2002. Bone marrow pathology established ALL (L1) in two and ALL (L2) in 7 patients. All patients had abnormal bone scans involving both appendicular and axial skeletons. These comprised axial skeleton in one, appendicular in seven and both appendicular and axial in one patient. Knowledge of scitigraphic changes acquired in the setting of ALL will expedite the early diagnosis of leukemia at presentation. The potential use of bone scintigraphy is important in that, the correct diagnosis is made on bone scan and confirmed by the examination of blood film, bone marrow aspirate and trephine.
https://ijms.sums.ac.ir/article_40128_acfe1af666aff938b3ddb278d6e99aba.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2015-10-03
29
2
97
99
40129
Tuberculous Involvement of the Thyroid
H.R. Abbasi
1
B. Geramizadeh
2
A. Sadeqholvaad
3
S. Sefidbakht
4
S. Bolandparvaz
5
Department of Surgery,
Shiraz University of Medical Sciences,
Shiraz, Iran
Department of Pathology,
Shiraz University of Medical Sciences,
Shiraz, Iran
Department of Endocrinology,
Shiraz University of Medical Sciences,
Shiraz, Iran
Department of Radiology,
Shiraz University of Medical Sciences,
Shiraz, Iran
Department of Surgery,
Shiraz University of Medical Sciences,
Shiraz, Iran
Despite increasing detection rates of various forms of extrapulmonary tuberculosis (TB), thyroid involvement with tubercle bacilli is rarely encountered and its imaging features have been reported in less than a handful of cases. Herein, we present the clinical, pathologic and imaging (ultrasound and CT scan) features of TB involvement of the thyroid gland in a 65-year-old woman who presented with a painful and rapidly enlarging thyroid mass. While reviewing fine needle aspiration results, the diagnosis of TB should be borne in mind, especially in the presence of imaging findings of a peripherally enhancing thyroid mass or thickening of the adjacent musculature and skin, the so-called dermal sign.
https://ijms.sums.ac.ir/article_40129_d0962287012c12034e59ad427d23c82c.pdf