eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
103
124
10.30476/ijms.2018.40516
40516
Review Article
Delayed Complications and Long-term Management of Sulfur Mustard Poisoning: Recent Advances by Iranian Researchers (Part І of ІІ)
Emadodin Darchini-Maragheh
1
Mahdi Balali-Mood
balalimmahdi@mums.ac.ir
2
Medical Toxicology Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Medical Toxicology Research Centre, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran
Chemical warfare agents are the most brutal weapons among the weapons of mass destruction. Sulfur mustard (SM) is a potent toxic alkylating agent known as “the King of the Battle Gases”. SM has been the most widely used chemical weapon during the wars. It was widely used in World War I. Thereafter, it was extensively employed by the Iraqi troops against the Iranian military personnel and even civilians in the border cities of Iran and Iraq in the period between 1983 and 1988. Long-term incapacitating properties, significant environmental persistence, lack of an effective antidote, and relative ease of manufacturing have kept SM a potential agent for both terrorist and military uses. Even 3 decades after SM exposure, numerous delayed complications among Iranian victims are still being reported by researchers. The most common delayed complications have been observed in the respiratory tracts of chemically injured Iranian war veterans. Also, skin lesions and eye disorders have been observed in most Iranian SM-exposed war veterans in the delayed phase of SM intoxication. Thus, extensive research has been conducted on Iranian war veterans during the past decades. Nevertheless, major gaps still continue to exist in the SM literature. Part І of this paper will discuss the delayed complications and manifestations of exposure to SM among Iranian victims of the Iran–Iraq conflict. Part ІІ, which will appear in the next issue of Iran J Med Sci, will discuss the long-term management and therapy of SM-exposed patients.
https://ijms.sums.ac.ir/article_40516_c1bba264289e47fdb53f0132eb445e61.pdf
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
125
139
10.30476/ijms.2018.40519
40519
Review Article
High Prevalence of Vitamin D Deficiency among Iranian Population: A Systematic Review and Meta-Analysis
Reza Tabrizi
kmsrc89@gmail.com
1
Mahmood Moosazadeh
mmoosazadeh1351@gmail.com
2
Maryam Akbari
m.akbari45@yahoo.com
3
Mohammad Hossein Dabbaghmanesh
4
Minoo Mohamadkhani
minoo.mohamadkhani@yahoo.com
5
Zatollah Asemi
asemi_z@yahoo.com
6
Seyed Taghi Heydari
heydari@yahoo.com
7
Mojtaba Akbari
akbari.epi@gmail.com
8
Kamran B Lankarani
9
Health Policy Research Center, Institute of Health, Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
Health Science Research Center, Addiction Institute, Mazandaran University of Medical Sciences, Sari, Iran
Health Policy Research Center, Institute of Health, Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
Endocrine and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Epidemiology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran
Research Center for Biochemistry and Nutrition in Metabolic Diseases, Kashan University of Medical Sciences, Kashan, Iran
Health Policy Research Center, Institute of Health, Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
Department of Epidemiology, Faculty of Health, Shiraz University of Medical Sciences, Shiraz, Iran
Health Policy Research Center, Institute of Health, Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran;
Background: The prevention and correction of vitamin D deficiency requires a precise depiction of the current situation and identification of risk factors in each region. The present study attempted to determine these entities using a systematic review and meta-analysis in Iran.Methods: Articles published online in Persian and English between 2000 and November 1, 2016, were reviewed. This was carried out using national databases such as SID, IranMedex, Magiran, and IranDoc and international databases such as PubMed, Google Scholar, and Scopus. The heterogeneity index among the studies was determined using the Cochran (Q) and I2 test. Based on the heterogeneity results, the random-effect model was applied to estimate the prevalence of vitamin D deficiency. In addition, meta-regression analysis was used to determine heterogeneity-suspected factors, and the Egger test was applied to identify publication bias.Results: The meta-analysis of 48 studies identified 18531 individuals with vitamin D deficiency. According to the random-effect model, the prevalence of vitamin D deficiency among male, female, and pregnant women was estimated to be 45.64% (95% CI: 29.63 to 61.65), 61.90% (95% CI: 48.85 to 74.96), and 60.45% (95% CI: 23.73 to 97.16), respectively. The results of the meta-regression analysis indicated that the prevalence of vitamin D deficiency was significantly different in various geographical regions (β=4.4; P=0.023).Conclusion: The results obtained showed a significant prevalence of vitamin D deficiency among the Iranian population, a condition to be addressed by appropriate planning.
https://ijms.sums.ac.ir/article_40519_bddbb66fd289d778d066f4defa465364.pdf
Vitamin D deficiency
Meta-analysis
Iran
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
140
149
10.30476/ijms.2018.40517
40517
Original Article(s)
Prevalence of Multiple Coronary Artery Disease Risk Factors in Kerman: A Population-Based Study in Southeast Iran
Hamid Najafipour
najafipourh@yahoo.co.uk
1
Mahdi Afshari
mahdiafshari99@gmail.com
2
Farzaneh Rostamzadeh
far_rostamzadeh@yahoo.com
3
Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran
Department of Epidemiology, School of Public Health, Zabol University of Medical Sciences, Zabol, Iran
Physiology Research Center, Institute of Basic and Clinical Physiology Sciences and Department of Physiology, Kerman University of Medical Sciences, Kerman, Iran
Background: The risk of disease with 1 risk factor is increased by the presence of additional risk factors. The goal of this study was to assess the prevalence of multiple coronary artery disease (CAD) risk factors among adults in Kerman, Iran, to identify the population groups most at risk.Methods: The present study included 5900 adults aged between 15 and 75 years in 2011 in Kerman, Iran. They were selected by 1-stage cluster sampling. Blood pressure, fasting blood glucose, lipids, and 6 CAD risk factors were assessed in the study population. Standardized prevalence rates were compared between the genders and age groups using the χ2 test. A P
https://ijms.sums.ac.ir/article_40517_b34f724ee314b6d33a682a01418076a9.pdf
Coronary artery disease
Risk factors
prevalence
Iran
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
150
157
10.30476/ijms.2018.40520
40520
Original Article(s)
From Negligence to Perception of Complexities in Adherence to Treatment Process in People with Diabetes: A Grounded Theory Study
Seyedeh Narjes Mousavizadeh
1
Tahereh Ashktorab
t_ashktorab@sbmu.ac.ir
2
Fazlollah Ahmadi
ahmadi_fazl@hotmail.com
3
Mitra Zandi
mitra.zandi@yahoo.com
4
Nursing and Midwifery School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Medical-Surgical Nursing, Nursing and Midwifery School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Department of Nursing, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Department of Medical-Surgical Nursing, Nursing and Midwifery School, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Background: Poor adherence of patients with type 2 diabetes to treatment is one of the most complex and important clinical concerns. It is the main issue of the present decade and acknowledged as a challenge to control and treat diabetes. This study was carried out to explore and understand how adherence to treatment process occurs among Iranian patients with type 2 diabetes.Methods: The present study is qualitative with grounded theory approach. The data were collected from December 2015 to July 2016 in Tehran (Iran) through individual semi-structured in-depth interviews, field notes, and memos from 21 patients with type 2 diabetes; combined with two members of their families and a healthcare professional. The data were analyzed based on Corbin and Strauss constant comparative analysis (2008).Results: Adherence to treatment is a transitional, interactive, and continuous process. For patients with diabetes, this process includes unperceived threat in diagnosis time (poor knowledge and skills, bottleneck of dependencies, superficial understanding of the new situation), bitter belief (downhill quality of life, physical and emotional treatment feedbacks), and adaptation to treatment (self-care dominance, regimen integration in daily activities). The process of adherence to treatment was influenced by knowledge and skill, social support, beliefs and values, psychological characteristics of people, and the nature of diabetes. Conclusion: Adherence to treatment in Iranian people with diabetes depends on the family and social context, which is challenging for the patient and leads to the negligence of health behaviors. It is vital for healthcare providers to identify these factors to encourage patients to adhere and commit to treatment in order to prevent irreversible complications of diabetes.
https://ijms.sums.ac.ir/article_40520_4605d0df79437039351724df8363683f.pdf
Adherence
Patient
diabetes mellitus
Grounded theory
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
158
163
10.30476/ijms.2018.40523
40523
Original Article(s)
The Effects of Size and Type of Vocal Fold Polyp on Some Acoustic Voice Parameters
Elaheh Akbari
akbari.phd5@yahoo.com
1
Sadegh Seifpanahi
panahi29@gmail.com
2
Ali Ghorbani
ghorbani.a@iums.ac.ir
3
Farzad Izadi
izadi@ent-hns.org
4
Farhad Torabinezhad
ftorabinezhad@yahoo.com
5
Department of Speech and Language Pathology, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran
Department of Speech and Language Pathology, School of Rehabilitation Sciences, Hamadan University of Medical Sciences, Hamadan, Iran
Department of Speech and Language Pathology, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran
ENT-Head and Neck Research Center, Hazrat Rasoul AKram Hospital, Iran University of Medical Sciences, Tehran, Iran
Department of Speech and Language Pathology, School of Rehabilitation Sciences, Iran University of Medical Sciences, Tehran, Iran
Background: Vocal abuse and misuse would result in vocal fold polyp. Certain features define the extent of vocal folds polyp effects on voice acoustic parameters. The present study aimed to define the effects of polyp size on acoustic voice parameters, and compare these parameters in hemorrhagic and non-hemorrhagic polyps.Methods: In the present retrospective study, 28 individuals with hemorrhagic or non-hemorrhagic polyps of the true vocal folds were recruited to investigate acoustic voice parameters of vowel/ æ/ computed by the Praat software. The data were analyzed using the SPSS software, version 17.0. According to the type and size of polyps, mean acoustic differences and correlations were analyzed by the statistical t test and Pearson correlation test, respectively; with significance level below 0.05. Results: The results indicated that jitter and the harmonics-to-noise ratio had a significant positive and negative correlation with the polyp size (P=0.01), respectively. In addition, both mentioned parameters were significantly different between the two types of the investigated polyps.Conclusion: Both the type and size of polyps have effects on acoustic voice characteristics. In the present study, a novel method to measure polyp size was introduced. Further confirmation of this method as a tool to compare polyp sizes requires additional investigations.
https://ijms.sums.ac.ir/article_40523_fbfd5a40e45e6f02a0681491de0273f2.pdf
Vocal cords
Polyps
Acoustics
Voice disorders
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
164
173
10.30476/ijms.2018.40525
40525
Original Article(s)
Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice
Wiwit Setyaningsih
wiwit.ananda.w.s@gmail.com
1
Nur Arfian
nur_arfian@ugm.ac.id
2
Efrayim Suryadi
efrayimsuryadi@yahoo.com
3
Muhammad Romi
4
Untung Tranggono
tranggonoun@yahoo.com
5
Dwi Sari
dwi.cahyani@ugm.ac.id
6
Department of Anatomy Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia
Department of Anatomy Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Pharmaco Street, North Sekip, Yogyakarta 55281, Indonesia
Background: Hyperuricemia contributes to kidney injury, characterized by tubular injury with epithelial–mesenchymal transition (EMT). Wnt5a/Ror2 signaling drives EMT in many kidney pathologies. This study sought to evaluate the involvement of Wnt5a/Ror2 in hyperuricemia-induced EMT in kidney tubular injury.Methods: A hyperuricemia model was performed in male Swiss background mice (3 months old, 30–40 g) with daily intraperitoneal injections of 125 mg/kg body weight (BW) of uric acid. The mice were terminated on day 7 (UA7, n=5) and on day 14 (UA14, n=5). Allopurinol groups (UAl7 and UAl14, each n=5) were added with oral 50 mg/kg BW of allopurinol treatment. The serum uric acid level was quantified, and tubular injury was assessed based on PAS staining. Reverse transcriptase-PCR was done to quantify Wnt5a, Ror2, E-cadherin, and vimentin expressions. IHC staining was done for E-cadherin and collagen I. We used the Shapiro–Wilk for normality testing and one-way ANOVA for variance analysis with a P
https://ijms.sums.ac.ir/article_40525_6d96ac3f398b941dd16064315fdef106.pdf
Hyperuricemia
Tubular injury
E-cadherin gene expression
Vimentin
Gene expression
Wnt5a/Ror2
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
174
183
10.30476/ijms.2018.40526
40526
Original Article(s)
Urtica Dioica Distillate Regenerates Pancreatic Beta Cells in Streptozotocin-Induced Diabetic Rats
Ali Gohari
1
Ali Noorafshan
noora@sums.ac.ir
2
Masoumeh Akmali
3
Fahimeh Zamani-Garmsiri
4
Atefeh Seghatoleslam
seghatolea@sums.ac.ir
5
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Histomorphometry and Stereology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Research Center for Traditional Medicine and History of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Background: Urtica dioica is known as an anti-hyperglycemic plant. Urtica dioica distillate (UD) is a traditional Iranian drink, locally known as “aragh gazaneh”. In spite of its widespread consumption in Iran, according to traditional Iranian medicine, there is no scientific report on the usefulness of UD for diabetic patients. This survey was designed to evaluate its protective effects for the recovery from diabetes by determining the serum insulin, blood glucose, volume of pancreatic islets, and the number and volume of β-cells in diabetic rats.Methods: A total of 48 Sprague-Dawley male rats (200-250 g) were randomly distributed into 6 groups (n=8), including non-diabetic plus distilled water (DW), non-diabetic plus UD, diabetic plus DW, diabetic plus UD, diabetic plus insulin, and diabetic plus glibenclamide. DW, UD, and glibenclamide were administered via intragastric gavage and insulin was injected subcutaneously. After four weeks of experiments, blood samples were collected for serum insulin and blood glucose assay. Pancreas was also evaluated using stereological method. The SPSS software was used for statistical analysis. Kruskal-Wallis, repeated measurements, and Mann-Whitney U test were applied for comparisons between the groups.Results: The treatment of diabetic rats with UD reduced the blood glucose dramatically (P<0.001) and increased serum insulin levels significantly (P=0.03) in comparison to the diabetic plus DW rats. Treatment with UD did not affect the mean β-cell volumes in the diabetic rats when compared to the diabetic plus DW rats, but the islet volumes and β-cell numbers were significantly recovered.Conclusion: UD treatment in diabetic rats improves hyperglycemia by partially restoring plasma insulin levels. The data suggest that UD prevents islet atrophy and/or regenerate pancreatic β-cells.
https://ijms.sums.ac.ir/article_40526_505e88fe91ac7aa2bd4f66c27ec25428.pdf
diabetes mellitus
Pancreatic beta cell
Urtica dioica distillate
Rats
Streptozotocin
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
184
194
10.30476/ijms.2018.40527
40527
Original Article(s)
The Preventive Role of Pioglitazone in Glycerol-Induced Acute Kidney Injury in Rats during Two Different Treatment Periods
Rama Mousleh
ramamosleh@hotmail.com
1
Shaza Al Laham
2
Ahmad Al-Manadili
dr.manadili@gmail.com
3
Departmentof Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University, Damascus, Syria
Departmentof Pharmacology and Toxicology, Faculty of Pharmacy, Damascus University, Damascus, Syria
Departmentof Oral Histopathology, Faculty of Dentistry, Damascus University, Damascus, Syria
Background: Acute kidney injury is the most life-threatening complication of rhabdomyolysis. Glycerol is commonly used to induce this injury. The aim was to investigate the renoprotective effects of pioglitazone and the possible advantage of administering the drug for a longer period.Methods: Twenty-four male Albino Wistar rats were randomly divided into 4 groups (n=6/group): (A) control, (B) glycerol (50%, 10 mL/kg intramuscularly), (C) glycerol+pioglitazone (10 mg/kg orally for 3 days), and (D) glycerol+pioglitazone (for 6 days). Serum urea and creatinine levels were measured to assess the renal function. Reduced glutathione (GSH) levels and histological alterations were also measured. Statistical analysis was performed using Prism (version 6). The numerical data were evaluated by ANOVA, followed by the Tukey tests. The categorical data were evaluated by the Mann–Whitney test and the Fisher exact tests. P
https://ijms.sums.ac.ir/article_40527_65eb06354fb89d5953bfcabcbd754edd.pdf
Kidney
Glycerol
Rhabdomyolysis
Acute kidney injury
Pioglitazone
Reduced glutathione
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
195
201
10.30476/ijms.2018.40528
40528
Original Article(s)
Amelioration of Pentylenetetrazole-Induced Seizures by Modulators of Sigma, N-Methyl-D-Aspartate, and Ryanodine Receptors in Mice
Mojtaba Keshavarz
moj.ph60@yahoo.com
1
Behdad Yekzaman
behdadyekzaman1990@gmail.com
2
Department of Pharmacology, Bushehr University of Medical Sciences, Bushehr, Iran; and Shiraz Neuroscience Research Center, Shiraz University of Medical Sciences, Shiraz, Iran;
School of Medicine, Bushehr University of Medical Sciences, Bushehr, Iran
Background: Sigma receptors, N-methyl-D-aspartate (NMDA) antagonist, and modulators of intracellular calcium may be useful for seizure control. Therefore, we aimed to evaluate the antiepileptic effects of opipramol, a sigma receptor agonist, against pentylenetetrazole (PTZ)-induced seizures in mice and assess ketamine and caffeine interaction with the antiepileptic effects of opipramol.Methods: PTZ (100 mg/kg) was used for the induction of seizure in 72 male albino Swiss strain of mice (n=8). Opipramole (10, 20, and 50 mg/kg), ketamine (50 mg/kg), caffeine (200 mg/kg), opipramole (20 mg/kg) plus ketamine (50 mg/kg), opipramole (20 mg/kg) plus caffeine (200 mg/kg), diazepam (5 mg/kg as a positive control), and the vehicle were administered interaperitoneally 30 minutes before the injection of PTZ. The latency was recorded for the clonic, tonic-clonic seizures, and death of animals after the injection of PTZ. Kruskal-Wallis test followed by Dunn’s test was used for the analysis of data. Statistical analysis was performed with the SPSS software version 23.0 and P
https://ijms.sums.ac.ir/article_40528_7a29df970b2c2a12996343eaa089bc41.pdf
Opipramol
Sigma
Pentylenetetrazole
Ketamine
Caffeine
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
202
207
10.30476/ijms.2018.40515
40515
Brief Report(s)
First Report on the Percentiles of the Glomerular Filtration Rate in Iranian Children Using the 2009 Schwartz Equations
Peyman Roomizadeh
p_roomizadeh@yahoo.com
1
Alaleh Gheissari
gheisari@gmail.com
2
Roya Kelishadi
kelishadi2009@gmail.com
3
Farhad Mahmoudi
mahmoudi.68@gmail.com
4
Amin Abedini
amin69.med@gmail.com
5
School of Medicine, Iran University of Medical Sciences, Tehran, Iran
Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran; and Department of Pediatric Nephrology, Isfahan University of Medical Sciences, Isfahan, Iran
Child Growth and Development Research Center, Department of Pediatrics, Isfahan University of Medical Sciences, Isfahan, Iran;
Medical Students’ Research Center, Isfahan University of Medical sciences, Isfahan, Iran
Isfahan Kidney Diseases Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
The glomerular filtration rate (GFR) is widely considered the best overall index of renal function. The Schwartz equations are designed for measuring the GFR in children between 1 and 16 years of age. In the present study, we investigated the percentiles of the GFR in a general population of Iranian children with no known renal disease via the 2009 Schwartz equations (updated and combined equations). Between 2010 and 2011, we selected 687 children aged 7–16 years from the Iranian province of Isfahan. Blood samples were analyzed for blood urea nitrogen, creatinine, and cystatin C. For each child, we calculated the GFR using 2 Schwartz equations. The Wilcoxon test was applied to examine the differences in the estimated GFRs between the equations. To determine the correlation between the GFRs obtained via the updated and combined Schwartz equations in the boys and the girls and also for the correlation between age and the GFR, we performed the Pearson or Spearman correlation coefficients. The statistical analyses were conducted using MedCalc, version 12.1.4.0 (MedCalc Software, Mariakerke, Belgium). The mean GFR was 100.06±19.80 mL/min/1.73 m2 based on the updated equation and 96.10±18.44 mL/min/1.73 m2 according to the combined equation. No significant differences were observed between these equations in estimating the GFRs. The correlation analysis for determining the association between age and the GFR estimated by the updated (r=0.05, P=0.1) and combined (r=0.06, P=0.09) Schwartz equations was not statistically significant. In conclusion, in the current study we showed that the updated and combined Schwartz equations exhibited high concordance in estimating GFR values in this age group.
https://ijms.sums.ac.ir/article_40515_0e9a68f0a0f105f967b4816204727554.pdf
Glomerular filtration rate
Pediatrics
Cystatin C
Iran
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
208
213
10.30476/ijms.2018.40521
40521
Brief Report(s)
Optimization of the Static Human Osteoblast/Osteoclast Co-culture System
James Jam Jolly
1
Kok-Yong Chin
imasoel@ppukm.ukm.edu.my
2
Mohd Fozi Nur Farhana
3
Ekram Alias
4
Kien Hui Chua
masoel@ppukm.ukm.edu.my
5
Wan Nuraini Wan Hasan
6
Soelaiman Ima-Nirwana
7
Departments of Pharmacology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Departments of Pharmacology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Departments of Pharmacology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Departments of Biochemistry, Faculty
of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Departments of Physiology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Departments of Pharmacology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Departments of Pharmacology, Faculty of Medicine, Pusat Perubatan University Kebangsaan Malaysia, Jalan Yaacob Latif, Bandar Tun Razak, 56000 Cheras, Wilayah Persekutuan Kuala Lumpur, Malaysia
Osteoblasts (OBs) and osteoclasts (OCs) are 2 major groups of bone cells. Their cell-to-cell interactions are important to ensure the continuity of the bone-remodeling process. Therefore, the present study was carried out to optimize an OB/OC co-culture system utilizing the human OB cell line hFOB 1.19 and OCs extracted from peripheral blood mononuclear cells (PBMNCs). It was a 2-step procedure, involving the optimization of the OB culture and the co-culture of the OBs with PBMNCs at an optimum ratio. Firstly, pre-OBs were cultured to 90% confluency and the time required for differentiation was determined. OB differentiation was determined using the van Gieson staining to detect the presence of collagen and Alizarin Red for calcium. Secondly, OBs and OCs were co-cultured at the ratios of 1 OC: 1 OB, 1 OC: 4 OBs, 2 OCs: 1 OB, and 1 OC: 2 OBs. Tartrate-resistant acid phosphatase (TRAP) staining was used to detect the differentiation of the OCs. The results showed that collagen was present on day 1, whereas calcium was detected as early as day 3. Based on the result of TRAP staining, 1 OC: 2 OBs was taken as the most appropriate ratio. No macrophage colony-stimulating factor and receptor activator of the nuclear factor-κB ligand were added because they were provided by the OBs. In conclusion, these optimization processes are vital as they ensure the exact time point and ratio of the OB/OC co-culture in order to produce a reliable and reproducible co-culture system.
https://ijms.sums.ac.ir/article_40521_06aa8a0a8bb6d72b5c959c44f7edbc09.pdf
Bone and Bones
Coculture Techniques
Osteoblasts
Osteoclasts
Bone Remodeling
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
214
217
10.30476/ijms.2018.40514
40514
Pulmonary Embolism as a Rare Complication of Liver Hydatid Cyst: Report of Two Cases
Omar Toumi
toumiomar2010@yahoo.com
1
Randa Salem
2
Sadok Ben Jabra
3
Ghedira Abdesslem
4
Faouzi Noomen
5
Abdel Aziz Hamdi
6
Department of General Surgery, Fattouma Bourguiba Hospital, Faculty of Medicine, University of Monastir, Monastir, Tunisia
Department of Radiology, Fattouma Bourguiba Hospital, Faculty of Medicine, University of Monastir, Monastir, Tunisia
Department of General Surgery, Fattouma Bourguiba Hospital, Faculty of Medicine, University of Monastir, Monastir, Tunisia
Department of General Surgery, Fattouma Bourguiba Hospital, Faculty of Medicine, University of Monastir, Monastir, Tunisia
Department of General Surgery, Fattouma Bourguiba Hospital, Faculty of Medicine, University of Monastir, Monastir, Tunisia
Department of General Surgery, Fattouma Bourguiba Hospital, Faculty of Medicine, University of Monastir, Monastir, Tunisia
Hydatid cyst is a helminth infection. The rupture of a hepatic hydatid cyst in inferior vena cava is a rare and lethal complication. Pulmonary embolism is the commonest manifestation. The diagnosis of hydatid cyst is made by histopathological or serological examination, and imaging may suggest the probability of hydatid cyst. Surgical treatment should be performed with caution and always under vascular control. We present two cases of hydatid cyst ruptured into the inferior vena cava. The patients also presented with dyspnea, hemoptysis, and intermittent cough at the time of admission. This presentation will be accompanied by a brief review of the literature. The radiological findings suggested the diagnosis. The first patient had a fatal hydatid pulmonary embolism and death occurred on the second day of hospitalization. The second patient refused surgery. These cases also exemplify the clinical and radiographic findings as well as the management of this complication.
https://ijms.sums.ac.ir/article_40514_7b97f1e1b1f7a678dba768809a2f96c7.pdf
Echinococcus
Echinococcosis
Pulmonary embolism
Vena cava
Inferior
Tomography
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
218
222
10.30476/ijms.2018.40518
40518
A New Mutation Causing Severe Infantile-Onset Pompe Disease Responsive to Enzyme Replacement Therapy
Hossein Moravej
drmoravej@yahoo.com
1
Anis Amirhakimi
a_amirhakimi@yahoo.com
2
Alireza Showraki
3
Hamid Amoozgar
amoozgarh@sums.ac.ir
4
Zahra Hadipour
dr.hodipour@yahoo.com
5
Ghasem Nikfar
dr_gh.nikfar@yahoo.com
6
Neonatal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Pediatrics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Pediatrics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Neonatal Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Medical Genetics, Sarem Cell Research Center and Hospital, Tehran, Iran
Department of Pediatrics, Fasa University of Medical sciences, Fasa, Iran
Pompe disease (PD), also known as “glycogen storage disease type II (OMIM # 232300)” is a rare autosomal recessive disorder characterized by progressive glycogen accumulation in cellular lysosomes. It ultimately leads to cellular damage. Infantile-onset Pompe disease (IOPD) is the most severe type of this disease and is characterized by severe hypertrophic cardiomyopathy and generalized hypotonia. Mutations in the acid alpha-glucosidase (GAA) gene, located at locus 17q25.3, are responsible for the disease leading to reduced activity of the acid alpha-glucosidase enzyme. To date, approximately 400 pathogenic mutations have been reported in the GAA gene. The aim of this study is to report a novel nonsense mutation in exon 4 of the GAA gene in an Iranian child suffering from IOPD. The patient was a female neonate with hypertrophic cardiomyopathy and a positive family history of IOPD. After definite diagnosis, enzyme-replacement therapy (ERT) was started for the patient, who was 2 months old. Now at the age of 20 months, she has had good growth and development and her echocardiographic parameters are within the normal range. This report shows that IOPD patients with this mutation can be treated with ERT successfully.
https://ijms.sums.ac.ir/article_40518_0ea089ce08791952f534e3214b2cb8ea.pdf
Enzyme replacement therapy
Glycogen storage disease type II
GAA protein
Human
Cardiomyopathy
Hypertrophy
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
223
226
10.30476/ijms.2018.40522
40522
Central Acinic Cell Carcinoma of the Mandible Simulating as Benign Odontogenic Lesion: A Case Report
Manas Bajpai
dr.manasbajpai@gmail.com
1
Nilesh Pardhe
pardhenilesh@gmail.com
2
Betina Chandolia
betina87@yahoo.co.in
3
Manika Arora
aroramanika07@gmail.com
4
Department of Oral and maxillofacial Pathology, NIMS Dental College, Jaipur, India
Department of Oral and maxillofacial Pathology, NIMS Dental College, Jaipur, India
Department of Oral and maxillofacial Pathology, NIMS Dental College, Jaipur, India
Department of Oral and maxillofacial Pathology, NIMS Dental College, Jaipur, India
Centrally occurring salivary gland tumors are rare. Because of a considerable overlap between the clinical and histopathological features, this group of tumors often produces a diagnostic difficulty to the clinicians and oral pathologists. Acinic cell carcinoma (ACC) is an unusual, low-grade, malignant salivary gland tumor that represents approximately 2% of the salivary gland tumors with almost 90% arising in the parotid gland. The rest involve the submandibular and the minor salivary gland. ACC of the jaw is extremely rare and, to our knowledge, only 8 cases have been reported in the English literature. Herein, a case of primary intraosseous ACC of the mandible in a 31-year-old woman is presented. The present case is unique, as the central ACC has never been reported in a patient in the third decade of life. The complete surgical removal of the tumor was carried out under general anesthesia along with the extraction of teeth #31, #32, #41, and #42. The follow-up period of 1-year was uneventful.
https://ijms.sums.ac.ir/article_40522_85c8221443b400c4f6bfdd7ef7c31b81.pdf
Carcinoma
Acinar cell
Central acinic cell carcinoma
Salivary gland neoplasms
Mandible
eng
Shiraz University of Medical Sciences
Iranian Journal of Medical Sciences
0253-0716
1735-3688
2018-03-01
43
2
227
230
10.30476/ijms.2018.40524
40524
Simultaneous Presence of Macular Corneal Dystrophy and Retinitis Pigmentosa in Three Members of a Family
Farhad Nejat
fanejat@yahoo.com
1
Hossein Aghamollaei
aghamolaei22@gmail.com
2
Shiva Pirhadi
shv_prhd@yahoo.com
3
Khosrow Jadidi
kh.jadidi@gmail.com
4
Mohammad Amin Nejat
5
Vision Health Research Center, Tehran, Iran
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
Department of Ophthalmology, Baqiyatallah University of Medical Sciences, Tehran, Iran
Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
Macular corneal dystrophy (MCD) is an autosomal recessive hereditary disease. In most cases, various mutations in carbohydrate sulfotransferase 6 (CHST6) gene are the main cause of MCD. These mutations lead to a defect in keratan sulfate synthesis. Retinitis pigmentosa (RP) is another eye disorder with nyctalopia as its common symptom. It has been shown that more than 65 genes have been implicated in different forms of RP. Herein, we report on a 9-member family with 2 girls and 5 boys. Both parents, one of the girls and one of the boys had normal eye vision and another boy had keratoconus. Other children (1 girl and 2 boys) suffered from both MCD and RP. Corneal transplantation and medical supplements were used for MCD and RP during the follow-up period, respectively. Based on the family tree, it seems that the inheritance of both diseases is autosomal recessive. Based on our search of databases, there is no report on the simultaneous presence of MCD and RP. To the best of our knowledge, the present article is the first case report on this topic. Molecular genetic investigation is needed to clarify the mechanism of concurrent MCD and RP.
https://ijms.sums.ac.ir/article_40524_33471711356764d3913fdad5c90781da.pdf
Macular corneal dystrophy
Retinitis pigmentosa
Pedigree
Genetic disease