ORIGINAL_ARTICLE
Association between Serum Vitamin D Level and Glycemic and Inflammatory Markers in Non-obese Patients with Type 2 Diabetes
Background: Low serum 25-hydroxy vitamin D (25(OH)D) has been shown to correlate with an increased risk of type 2 diabetes mellitus (T2DM). The objective of this study was to investigate the association between serum 25(OH)D and glycemic and inflammatory markers in non-obese patients with T2DM. Methods: Eighty-four non-obese patients with T2DM were recruited in this cross-sectional study. Demographic, anthropometric, and dietary information was obtained from all the participants. The serum concentrations of glucose, HbA1C, insulin, 25(OH)D, and inflammatory markers including tumor necrosis factor-alpha (TNF-α) and high sensitive C-reactive protein (hs-CRP) were measured. A homeostatic model of insulin resistance (HOMA-IR) was also evaluated.Results: The mean serum concentration of 25(OH)D was 11.01±5.55 ng/mL. Severe deficiency, deficiency, and insufficiency of vitamin D were detected in 60.71%, 35.72%, and 3.57% of the participants, respectively. The results showed that those in the lowest group of serum 25(OH)D had significantly higher TNF-α than did those in the highest group (P=0.026). Although the association between serum 25(OH)D and fasting blood sugar and TNF-α was statistically significant (P=0.049 and P=0.044, respectively), the other glycemic markers and hs-CRP did not have any significant relationships with 25(OH)D. Conclusion: According to the high prevalence of vitamin D deficiency in the diabetic patients and the inverse relationship between serum 25(OH)D and fasting blood sugar and TNF-α in this study, vitamin D status may be a determining factor of systemic inflammation in patients with T2DM. Further studies with larger sample sizes are suggested in this regard.
https://ijms.sums.ac.ir/article_40381_a2e5ae25fe18c68fd8c9bd7e88ac26f3.pdf
2016-09-01
367
373
diabetes mellitus
Type 2
Inflammatory factors
Obesity
Vitamin D
Fatemeh
Haidari
haidari58@gmail.com
1
Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
Mehrnoosh
Zakerkish
zakerky.m@yahoo.com
2
Health Research Institute, Diabetes Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
Majid
Karandish
mkarandish@yahoo.com
3
Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
AUTHOR
Azadeh
Saki
saki-a@ajums.ac.ir
4
Department of Biostatistics, Faculty of Medical Science, Ahvaz Jundishapur University of Medical sciences, Ahvaz, Iran
AUTHOR
Sakineh
Pooraziz
s.pooraziz@yahoo.com
5
Nutrition and Metabolic Diseases Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Role of the Internal Superior Laryngeal Nerve in the Motor Responses of Vocal Cords and the Related Voice Acoustic Changes
Background: Repeated efforts by researchers to impose voice changes by laryngeal surface electrical stimulation (SES) have come to no avail. This present pre-experimental study employed a novel method for SES application so as to evoke the motor potential of the internal superior laryngeal nerve (ISLN) and create voice changes.Methods: Thirty-two normal individuals (22 females and 10 males) participated in this study. The subjects were selected from the students of Iran University of Medical Sciences in 2014. Two monopolar active electrodes were placed on the thyrohyoid space at the location of the ISLN entrance to the larynx and 1 dispersive electrode was positioned on the back of the neck. A current with special programmed parameters was applied to stimulate the ISLN via the active electrodes and simultaneously the resultant acoustic changes were evaluated. All the means of the acoustic parameters during SES and rest periods were compared using the paired t-test.Results: The findings indicated significant changes (P=0.00) in most of the acoustic parameters during SES presentation compared to them at rest. The mean of fundamental frequency standard deviation (SD F0) at rest was 1.54 (SD=0.55) versus 4.15 (SD=3.00) for the SES period. The other investigated parameters comprised fundamental frequency (F0), minimum F0, jitter, shimmer, harmonic-to-noise ratio (HNR), mean intensity, and minimum intensity.Conclusion: These findings demonstrated significant changes in most of the important acoustic features, suggesting that the stimulation of the ISLN via SES could induce motor changes in the vocal folds. The clinical applicability of the method utilized in the current study in patients with vocal fold paralysis requires further research.
https://ijms.sums.ac.ir/article_40386_de8eaa7f164ffd1fecd130a23e967ae2.pdf
2016-09-01
374
381
Laryngeal nerves
Electric stimulation
Voice
Acoustics
Vocal cords
Sadegh
Seifpanahi
panahi29@gmail.com
1
Department of Speech and Language
Pathology, School of Rehabilitation
Sciences, Iran University of Medical
Sciences, Tehran, Iran
AUTHOR
Farzad
Izadi
izadi@ent-hns.org
2
Division of Laryngology, Rasoul Akram
Medical Complex, Iran University of
Medical Sciences, Tehran, Iran
AUTHOR
Ali-Ashraf
Jamshidi
alijamshidipt@gmail.com
3
Department of Physical Therapy,
School of Rehabilitation Sciences, Iran
University of Medical Sciences, Tehran,
Iran
LEAD_AUTHOR
Farhad
Torabinezhad
ftorabinezhad@yahoo.com
4
Department of Speech and Language
Pathology, School of Rehabilitation
Sciences, Iran University of Medical
Sciences, Tehran, Iran
AUTHOR
Javad
Sarrafzadeh
j.sarrafzadeh@gmail.com
5
Department of Physical Therapy,
School of Rehabilitation Sciences, Iran
University of Medical Sciences, Tehran,
Iran
AUTHOR
Siavash
Mohammadi
samansiavash@gmail.com
6
Department of Speech and Language
Pathology, School of Rehabilitation
Sciences, Iran University of Medical
Sciences, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Neural Stem Cell-based Intraocular Administration of Pigment Epithelium-derived Factor Promotes Retinal Ganglion Cell Survival and Axon Regeneration after Optic Nerve Crush Injury in Rat: An Experimental Study
Background: Pigment epithelium-derived factor (PEDF) is regarded as a multifunctional protein possessing neurotrophic and neuroprotective properties. PEDF has a very short half-life, and it would require multiple injections to maintain a therapeutically relevant level without a delivery system. However, multiple injections are prone to cause local damage or infection. To overcome this, we chose a cell-based system that provided sustained delivery of PEDF and compared the effect of weekly injections of PEDF and neural stem cell (NSC)-based intraocular administration of PEDF on retinal ganglion cell (RGC) survival and axon regeneration after optic nerve injury.Methods: Seventy-two rats were randomly assigned to 3 groups: group with injections of phosphate buffered saline (PBS) (n=24), group with weekly injections of PEDF (n=24), and group with NSC-based administration of PEDF (n=24). Western blot was used to analyze the PEDF protein level 2 weeks after injection. Retinal flat mounts and immunohistochemistry were employed to analyze RGC survival and axon regeneration 2 weeks and 4 weeks after injection. The data were analyzed with one-way ANOVA in SPSS (version 19.0). A p
https://ijms.sums.ac.ir/article_40390_f0b3157ab13bfa799fc69620f4858560.pdf
2016-09-01
382
390
Pigment epithelium-derived factor
Neural stem cells
Optic nerve
Wei-Min
Zhang
1
Department of Neurosurgery, Zhumadian Central Hospital, Zhumadian, China
AUTHOR
Zhi-Ren
Zhang
chgn3619@163.com
2
Department of Medical Administration, Zhumadian Central Hospital, Zhumadian, China
LEAD_AUTHOR
Yong-Gang
Zhang
3
Department of Neurosurgery, Zhumadian Central Hospital, Zhumadian, China
AUTHOR
Yan-Sheng
Gao
4
Department of Neurosurgery, Zhumadian Central Hospital, Zhumadian, China
AUTHOR
ORIGINAL_ARTICLE
Effect of Cyperus Rotundus on Cytokine Gene Expression in Experimental Inflammatory Bowel Disease
Background: The protective effect of the chloroform extract of Cyperus rotundus (CHCR) is attributed to its anti-inflammatory and antioxidant activities. Cytokines, important regulators of inflammation and repair, play a key role in the pathogenesis of inflammatory bowel disease (IBD). Targeting these cytokines can effectively ameliorate the symptoms of IBD. The aim of the present study was to unravel the molecular mechanism through cytokine regulation in rats in experimental IBD.Methods: Sprague Dawley rats were randomly allocated to 5 groups (n=6). Group I served as the normal control. Group II served as the vehicle control and received 50% ethanol intracolonically on day 11 of the study. Group III served as the model control. Group IV and Group V were given standard drug 5-aminosalicylic acid (100 mg/kg) and CHCR (800 mg/kg), respectively, for 18 days once a day orally. Colitis was induced with dinitrobenzene sulfonic acid (180 mg/kg in 50% ethanol) intracolonically in groups III–V on day 11 of the study. On day 18, the rats were euthanized and colon tissues were removed for IL-4, IL-6, IL-12, and IFN-gamma gene expression studies using quantitative RT-PCR.Results: The expression levels of proinflammatory cytokines IL-4, IL-6, IL-12, and IFN-gamma were upregulated in the model control rats. Pretreatment with 5-aminosalicylic acid (100 mg/kg) and CHCR (800 mg/kg) significantly decreased the fold of the expression of the above cytokines.Conclusion: CHCR acts as a molecular brake and downregulates the expression of proinflammatory cytokine genes; this is beneficial for reducing the severity of the experimental IBD. Thus, Cyperus rotundus is a safe, economical, and effective alternative for the treatment of patients with IBD.
https://ijms.sums.ac.ir/article_40393_f358fd4831d9e22f3de483d1fe0defda.pdf
2016-09-01
391
398
Inflammatory bowel diseases
Cyperus rotundus
Interleukin-4
Interleukin-6
Interleukin-12
Interleukin-18
Sarika
Johari
sarikajohari2008@gmail.com
1
Department of Pharmacology, Anand Pharmacy College, Anand, Gujarat, India;
LEAD_AUTHOR
Chaitanya
Joshi
2
Department of Animal Biotechnology,
College of Veterinary Science and
Animal Husbandry, Anand Agricultural
University, Anand, Gujarat, India
AUTHOR
Tejal
Gandhi
3
Department of Pharmacology,
Anand Pharmacy College, Anand,
Gujarat, India;
AUTHOR
ORIGINAL_ARTICLE
SDS-PAGE Analysis of the Outer Membrane Proteins of Uropathogenic Escherichia coli Isolated from Patients in Different Wards of Nemazee Hospital, Shiraz, Iran
Background: Outer membrane proteins (OMPs) constitute the main structure and about half of the cell wall of Gram-negative bacteria. The OMPs of Escherichia coli (E. coli) play an important role in its drug resistance. Previous studies have shown that the OMPs of E. coli enhance its pathogenic effects by helping the bacterium to evade the immune defense and promote its adsorption to host cells. We sought to compare E. coli isolates collected from different hospital wards and to perform a primary investigation of the association between the serotypes and profiles of their OMPs. We also aimed to detect the diversity of the E. coli isolates from the hospitalized patients. Methods: A total of 115 isolates of E. coli were collected from patients hospitalized in Nemazee Hospital, Shiraz, Iran. After biochemical and serological tests, OMPs were extracted by using glass beads and N-Lauroylsarcosine sodium. OMP typing was done by 10% SDS-PAGE and Coomassie brilliant blue staining. In terms of the number of protein bands, OMP-I was detected with 2 bands, OMP-α with 3 bands, and OMP-β with1 band. Results: Of the 115 isolates, 103 were OMP-I and 12 were OMP-α; none of the isolates belonged to OMP-β. Our statistical analyses showed a relationship between OMP patterns and other factors, including hospital wards and source of samples. Serotyping showed that the majority of the isolates were O128. Conclusion: Our results demonstrated some similarities between the OMP band patterns of the analyzed groups of E. coli. Of all the OMPs in the isolates from the hospitalized and outpatient department patients, OmpA and OmpC were the most prevalent proteins in the outer membrane of the studied uropathogenic E. coli.
https://ijms.sums.ac.ir/article_40394_3eff5105ffc9d59818a6e4afebb9b746.pdf
2016-09-01
399
405
Escherichia coli
Bacterial outer membrane proteins
Urinary tract infections
Electrophoresis
Polyacrylamide gel
Bacterial typing techniques
Behzad
Dehghani
dehghani_behzad@gmail.com
1
Department of Bacteriology and Virology,
School of Medicine, Shiraz University of
Medical Sciences, Shiraz, Iran
AUTHOR
Mohammad
Mottamedifar
2
Department of Bacteriology and Virology,
School of Medicine, Shiraz University of
Medical Sciences, Shiraz, Iran; and Shiraz HIV/AIDS Research Center Shiraz
University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
Hossein
Khoshkharam-Roodmajani
med_bactori@sums.ac.ir
3
Department of Bacteriology and Virology,
School of Medicine, Shiraz University of
Medical Sciences, Shiraz, Iran;
AUTHOR
Amir
Hassanzadeh
amirs66@gmail.com
4
Department of Bacteriology and Virology,
School of Medicine, Shiraz University of
Medical Sciences, Shiraz, Iran
AUTHOR
Kamyar
Zomorrodian
zomorodian@sums.ac.ir
5
Basic Sciences in Infectious Disease
Research Center, School of Medicine, Shiraz
University of Medical Sciences, Shiraz, Iran
AUTHOR
Amir
Rahimi
amirrahimi@sums.ac.ir
6
Department of Molecular Medicine,
School of Advanced Medical Science and
Technology, Shiraz University of Medical
Sciences, Shiraz, Iran
AUTHOR
ORIGINAL_ARTICLE
In Silico Analysis of Glutaminase from Different Species of Escherichia and Bacillus
Background: Glutaminase (EC 3.5.1.2) catalyzes the hydrolytic degradation of L-glutamine to L-glutamic acid and has been introduced for cancer therapy in recent years. The present study was an in silico analysis of glutaminase to further elucidate its structure and physicochemical properties.Methods: Forty glutaminase protein sequences from different species of Escherichia and Bacillus obtained from the UniProt Protein Database were characterized for homology search, physiochemical properties, phylogenetic tree construction, motif, superfamily search, and multiple sequence alignment. Results: The sequence level homology was obtained among different groups of glutaminase enzymes, which belonged to superfamily serine-dependent β-lactamases and penicillin-binding proteins. The phylogenetic tree constructed indicated 2 main clusters for the glutaminases. The distribution of common β-lactamase motifs was also observed; however, various non-common motifs were also observed.Conclusion: Our results showed that the existence of a conserved motif with a signature amino-acid sequence of β-lactamases could be considered for the genetic engineering of glutaminases in view of their potential application in cancer therapy. Nonetheless, further research is needed to improve the stability of glutaminases and decrease their immunogenicity in both medical and food industrial applications.
https://ijms.sums.ac.ir/article_40395_fba50553dfe54d0a53bfceb591e88f40.pdf
2016-09-01
406
414
Escherichia
Bacillus
Glutaminase
Computer simulation
Cambyz
Irajie
1
Department of Public Health and Food
Hygiene, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
AUTHOR
Milad
Mohkam
2
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Navid
Nezafat
navidnezafat@yahoo.com
3
Pharmaceutical Sciences Research
Center, Shiraz University of Medical
Sciences, Shiraz, Iran
AUTHOR
Saeed
Hosseinzadeh
4
Department of Public Health and Food
Hygiene, School of Veterinary Medicine,
Shiraz University, Shiraz, Iran
AUTHOR
Mahmood
Aminlari
aminlari@shirazu.ac.ir
5
Department of Biochemistry, School of
Veterinary Medicine, Shiraz University,
Shiraz, Iran
AUTHOR
Younes
Ghasemi
ghasemiy@sums.ac.ir
6
Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Pharmaceutical Biotechnology, School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran; and Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Auditory Brainstem Response Wave Amplitude Characteristics as a Diagnostic Tool in Children with Speech Delay with Unknown Causes
Speech delay with an unknown cause is a problem among children. This diagnosis is the last differential diagnosis after observing normal findings in routine hearing tests. The present study was undertaken to determine whether auditory brainstem responses to click stimuli are different between normally developing children and children suffering from delayed speech with unknown causes. In this cross-sectional study, we compared click auditory brainstem responses between 261 children who were clinically diagnosed with delayed speech with unknown causes based on normal routine auditory test findings and neurological examinations and had >12 months of speech delay (case group) and 261 age- and sex-matched normally developing children (control group). Our results indicated that the case group exhibited significantly higher wave amplitude responses to click stimuli (waves I, III, and V) than did the control group (P=0.001). These amplitudes were significantly reduced after 1 year (P=0.001); however, they were still significantly higher than those of the control group (P=0.001). The significant differences were seen regardless of the age and the sex of the participants. There were no statistically significant differences between the 2 groups considering the latency of waves I, III, and V. In conclusion, the higher amplitudes of waves I, III, and V, which were observed in the auditory brainstem responses to click stimuli among the patients with speech delay with unknown causes, might be used as a diagnostic tool to track patients’ improvement after treatment.
https://ijms.sums.ac.ir/article_40382_cf013ebc0a943630302f8ee83c5b9d58.pdf
2016-09-01
415
421
Language development disorders
diagnosis
Differential
Evoked potentials
Auditory
Brain stem
Susan
Abdi
susanabdi2000@gmail.com
1
Arash Hospital, Tehran University of Medical Sciences, Tehran, Iran
LEAD_AUTHOR
Ghamartaj
Khanbabaee
2
Mofid Children’s Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
Kourosh
Sheibani
3
Imam Hossein Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
AUTHOR
ORIGINAL_ARTICLE
Prevalence of the Most Common Virulence-Associated Genes among Brucella Melitensis Isolates from Human Blood Cultures in Hamadan Province, West of Iran
Brucellosis is a widespread zoonotic disease causing considerable economic and public health problems. Despite animal vaccination, brucellosis remains endemic in some areas such as Iran, especially in the western Iranian province of Hamadan. We sought to detect some of the most common virulence-associated genes in Brucella isolated from human blood cultures to determine the prevalence of some virulence genes among Brucella isolates. Fifty-seven isolates were studied from patients with a clinical diagnosis of brucellosis who referred to the Infectious Diseases Ward of Sina Hospital in Hamadan Province, Iran, between April 2013 and July 2014. Blood samples were collected for the diagnosis of brucellosis using the BACTEC blood culture system. All of these isolates were confirmed by the bcsp31 Brucella-specific gene. We detected 11 virulence-associated genes of Brucella, namely cβg, virB, znuA, ure, bvfA, omp25, omp31, wbkA, mviN, manA, and manB, which are important for the pathogenesis of this bacterium in the intracellular environment by multiplex PCR. Totally, 149 patients with a clinical diagnosis of brucellosis were enrolled in this study. Fifty-seven (38.3%) patients had positive blood cultures. On biochemical and molecular testing, all of the isolates were Brucella melitensis. Ten of the virulence genes were detected among all of the 57 isolates, but the bvf gene was detected in 53 (93%) isolates. The high prevalence of virulence-associated genes among the Brucella isolates detected in Hamadan Province, Iran, underscores the pathogenicity of this bacterium in this region.
https://ijms.sums.ac.ir/article_40387_fc046ee5792da523e71b66d1413adccb.pdf
2016-09-01
422
429
Virulence factors
Brucella melitensis
Brucellin
Iran
Zahra
Naseri
zahra_naseri62@yahoo.com
1
Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Mohammad Yousef
Alikhani
alikhani43@yahoo.com
2
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Seyed Hamid
Hashemi
hashemi@umsha.ac.ir
3
Brucellosis Research Center, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Farideh
Kamarehei
kamerehei@yahoo.com
4
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
AUTHOR
Mohammad Reza
Arabestani
5
Department of Microbiology, Faculty of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Effects of Lycopene and Sodium Valproate on Pentylenetetrazol-Induced Kindling in Mice
Sodium valproate and tomato extract have been studied in different experimental models of epilepsy individually. The aim of the present study was to evaluate the effect of lycopene on the antiepileptic effects of sodium valproate against pentylenetetrazol-induced kindling in mice. Swiss albino mice of either sex were randomly divided into 5 groups, with each group containing 8 mice. These groups were treated with pentylenetetrazol (45 mg/kg on days 8, 10, and 12 and 70 mg/kg on day 14 day, i.p.); sodium valproate (200 mg/kg, p.o.) + pentylenetetrazol; lycopene (2 mg/kg, p.o.) + sodium valproate (200 mg/kg, p.o.) + pentylenetetrazol; and lycopene (4 mg/kg, p.o.) + sodium valproate (200 mg/kg, p.o.) + pentylenetetrazol, for 14 days, respectively. After treatment, the animals were observed for 30 minutes for behavioral analysis. Subsequently, the animals were sacrificed, and their brain was removed for the biochemical estimations of thiobarbituric acid reactive substances, catalase, superoxide dismutase activity, reduced glutathione, and gamma-aminobutyric acid. Significant pentylenetetrazol-induced seizure was characterized by alteration in the seizure score and latency as well as a significant increase in the levels of brain thiobarbituric acid reactive substances and a significant decrease in reduced glutathione, catalase, superoxide dismutase, and gamma-aminobutyric acid levels. Treatment with sodium valproate and lycopene significantly restored the seizure score, latency, thiobarbituric acid reactive substance, reduced glutathione, catalase, superoxide dismutase, and gamma-aminobutyric acid levels near to normal compared to pentylenetetrazol. The present study provides experimental evidence that a combination therapy of lycopene along with sodium valproate attenuated seizure and oxidative stress against pentylenetetrazol-induced kindling in mice.
https://ijms.sums.ac.ir/article_40391_1f30f232ddfaf25313c8d66ed3b75c8f.pdf
2016-09-01
430
436
Kindling
Neurologic
Lycopene
Pentylenetetrazol
Epilepsy
Gamma-aminobutyric acid
Vinay
Kumar
vinaykumarpatel@gmail.com
1
Department of Pharmacology, School of Pharmacy, Krishna Institute of Engineering and Technology, Ghaziabad (UP), India
LEAD_AUTHOR
Sandeep Kumar
Sharma
sandeepshrm040@gmail.com
2
Department of Pharmacology, School of Pharmacy, Krishna Institute of Engineering and Technology, Ghaziabad (UP), India
AUTHOR
Kandassamy
Nagarajan
rknagaraaja@gmail.com
3
Department of Pharm. Chemistry, School of Pharmacy, Krishna Institute of Engineering and Technology, Ghaziabad (UP), India
AUTHOR
Praveen Kumar
Dixit
praveen.dixit@kiet.edu
4
Department of Pharmacology, School of Pharmacy, Krishna Institute of Engineering and Technology, Ghaziabad (UP), India
AUTHOR
ORIGINAL_ARTICLE
Glaucoma Surgery in Pregnancy: A Case Series and Literature Review
AbstractGlaucoma management in pregnant patients is a real challenge, especially when the glaucoma is not controlled with medications. We report the results of 6 incisional glaucoma surgeries for the management of medically uncontrolled glaucoma patients during pregnancy. This retrospective, case series was conducted on the 6 eyes of 3pregnant patients with uncontrolled glaucoma using maximum tolerable medications. Details of the glaucoma surgical management of these patients as well as their postoperative care and pregnancy and clinical outcomes on longitudinal follow-up are discussed. All 3 patients had juvenile open-angle glaucoma and were on various anti-glaucoma medications, including oral acetazolamide. The first case described underwent trabeculectomy without antimetabolites in both eyes because of uncontrolled intraocular pressure with topical medications. The surgery was done with topical lidocaine jelly and subconjunctival lidocaine during the second and third trimesters. The second patient had an Ahmed valve implantation in both eyes during the second and third trimesters because of uncontrolled IOP with topical medications and no response to selective laser trabeculoplasty. Surgery was done with topical tetracaine and subconjunctival and sub-Tenon’s lidocaine. The third case had a Baerveldt valve implantation under general anesthesia in the second trimester. In selected pregnant glaucoma patients with medically uncontrolled intraocular pressure threatening vision, incisional surgery may lead to good outcomes for the patient with no risk for the fetus.
https://ijms.sums.ac.ir/article_40383_7921121ee57fb890fdcfdc80e54d040f.pdf
2016-09-01
437
445
Glaucoma drainage implants
Pregnancy
Trabeculectomy
Mohammad Reza
Razeghinejad
razeghinejad@gmail.com
1
Poostchi Eye Research Center,
Poostchi Clinic, Shiraz University of
Medical Sciences, Shiraz, Iran;
LEAD_AUTHOR
Masoumeh
Masoumpour
masoumpour@yahoo.com
2
Poostchi Eye Research Center,
Poostchi Clinic, Shiraz University of
Medical Sciences, Shiraz, Iran;
AUTHOR
Mohammad Hossein
Eghbal
eghbalmh@sums.ac.ir
3
Department of Anesthesiology, Faghihi
Hospital, Shiraz University of Medical
Sciences, Shiraz, Iran
AUTHOR
Jonathan S.
Myers
4
Glaucoma Service, Wills Eye Institute,
Jefferson Medical College, Philadelphia,
USA
AUTHOR
Marlene R.
Moster
5
Glaucoma Service, Wills Eye Institute,
Jefferson Medical College, Philadelphia,
USA
AUTHOR
ORIGINAL_ARTICLE
Huge Parathyroid Adenoma with Dysphagia Presentation; A Case Report from Southern Iran
AbstractParathyroid adenoma is a benign tumor of the parathyroid glands. The cause of most parathyroid adenomas is unknown. Parathyroid adenoma increases the secretion of parathyroid hormone and results in primary hyperparathyroidism. High amounts of parathyroid hormone in the blood cause the imbalance of calcium, which leads to various complications such as kidney stones, depression, lethargy, nausea, vomiting, abdominal pain, myalgia, bone and joint pain, hoarseness, etc. Oropharyngeal dysphagia is defined as having problem in swallowing due to abnormalities in the structure and function of oropharynx and other related organs. The exact prevalence of dysphagia caused by parathyroid adenoma is unknown, but since this complication can lead to increased mortality and morbidity, its diagnosis is important. It is difficult to distinguish parathyroid malignancies from parathyroid adenoma even after surgery. Therefore, the final diagnosis is possible through surgery and histopathological evaluation. Here, a case of parathyroid adenoma with first presentation of generalized weakness and dysphagia has been reported.
https://ijms.sums.ac.ir/article_40384_efb627361d9f4108ed989458a869e884.pdf
2016-09-01
446
449
Parathyroid neoplasms
Dysphagia
Weakness
Iran
Bizhan
Ziaeean
ziaianb@sums.ac.ir
1
Department of General Surgery, Shiraz University of Medical Sciences, Shiraz, Iran
AUTHOR
Sahar
Sohrabi-Nazari
2
Department of General Surgery, Shiraz University of Medical Sciences, Shiraz, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Cesarean Scar Pregnancy Managed with Conservative Treatment
Cesarean scar pregnancy (CSP) is a rare, but life-threatening type of ectopic pregnancy. An exact and early diagnosis of CSP is very important for prognosis. The aim of the present study was to describe 4 women with CSP and discuss their clinical presentations, diagnoses, and various management options along with the published literature. Four women with a suspicion of CSP or cervical pregnancy were referred to our hospital between August 2013 and January 2014. All the patients were counseled about medical management options. After the treatment, serum beta human chorionic gonadotropin (ß-hCG) levels were followed weekly until they reached
https://ijms.sums.ac.ir/article_40388_4224ecbeca8eb9a41ae2f43c9f846d6c.pdf
2016-09-01
450
455
Cesarean scar pregnancy
Ectopic pregnancy
Methotrexate
Treatment
Aysen
Boza
aysenboza@hotmail.com
1
Goztepe Research and Training Hospital, Kadikoy, Istanbul;
LEAD_AUTHOR
Barıs
Boza
barisboza@hotmail.com
2
Zeynep Kamil Maternity and Children
Research, Training Hospital, Uskudar,
Istanbul, Turkey
AUTHOR
Murat
Api
muratapi@hotmail.com
3
Zeynep Kamil Maternity and Children
Research, Training Hospital, Uskudar,
Istanbul, Turkey
AUTHOR
ORIGINAL_ARTICLE
A Novel Missense Mutation in CLCN1 Gene in a Family with Autosomal Recessive Congenital Myotonia
Congenital recessive myotonia is a rare genetic disorder caused by mutations in CLCN1, which codes for the main skeletal muscle chloride channel ClC-1. More than 120 mutations have been found in this gene. The main feature of this disorder is muscle membrane hyperexcitability. Here, we report a 59-year male patient suffering from congenital myotonia. He had transient generalized myotonia, which started in early childhood. We analyzed CLCN1 sequence in this patient and other members of his family. We found a new missense mutation in CLCN1 gene (c.1886T>C, p.Leu629Pro). Co-segregation of this mutation with the disease was demonstrated by direct sequencing of the fragment in affected as well as unaffected members of this family. In addition, in silico analyses predicted that this nucleotide change would impair the protein function. Thus, this new nucleotide variation can be used for prenatal diagnosis in this family.
https://ijms.sums.ac.ir/article_40392_c81571ca613ab39bd3f37377020dc01f.pdf
2016-09-01
456
458
Myotonia congenita
CLCN1 protein
Mutation
Mohammad
Miryounesi
miryounesi@gmail.com
1
Genomic Research Center, Shahid
Beheshti University of Medical Sciences,
Tehran, Iran;
AUTHOR
Soudeh
Ghafouri-Fard
ghafourifard@razi.tums.ac.ir
2
Department of Medical Genetics,
Shahid Beheshti University of Medical
Sciences, Tehran, Iran;
AUTHOR
Majid
Fardaei
fardaeim@sums.ac.ir
3
Department of Medical Genetics,
Shiraz University of Medical Sciences,
Shiraz, Iran
LEAD_AUTHOR
ORIGINAL_ARTICLE
Effect of Ramadan Fast on Liver Function Tests
https://ijms.sums.ac.ir/article_40385_ece865cb84f32824b15cc20f5a9b236a.pdf
2016-09-01
459
460
Jafar
Nasiri
jhnasiri@yahoo.com
1
Department of Internal Medicine, Faculty of Medicine, Shaharekord University of Medical Sciences, Shahrekord, Iran;
LEAD_AUTHOR
Soleiman
Kheiri
kheiri.soleiman@gmail.com
2
Department of Epidemiology and Biostatistics, Faculty of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran;
AUTHOR
Abolfazl
Khoshdel
nikakhosh@gmail.com
3
Department of Pediatrics, Faculty of Medicine, Shahrekord University of Medical Sciences, Shahrekord, Iran;
AUTHOR
Amirgholi
Jafari Boroujeni
jafariborojeni@yahoo.com
4
Islamic Medicine Research Committee, Shahrekord University of Medical Sciences, Shahrekord, Iran
AUTHOR
ORIGINAL_ARTICLE
Associations of Suicide Seasonality with Rural- Urban Residence and Mental Disorders in Ilam, Iran
https://ijms.sums.ac.ir/article_40389_285cdec50e2e2ef0cfc3a1a7aa176c3b.pdf
2016-09-01
461
462
Yousef
Veisani
yousefveisani@yahoo.com
1
Psychosocial Injuries Research Center, Ilam University of Medical Sciences, Ilam, Iran
AUTHOR
Ali
Delpisheh
alidelpisheh@yahoo.com
2
Department of Clinical Epidemiology, Ilam University of Medical Sciences, Ilam, Iran
LEAD_AUTHOR
Kourosh
Sayehmiri
sayehmiri@razi.tams.ac.ir
3
Department of Social Medicine, Ilam University of Medical sciences, Ilam, Iran
AUTHOR
Ghobad
Moradi
moradi_gh@yahoo.com
4
Social Determinants of Health Research Center, Kurdistan University of Medical Sciences, Sanandaj, Iran
AUTHOR
Jafar
Hassanzadeh
jhasanzad@sums.ac.ir
5
Research Center for Health Sciences, Department of Epidemiology, School of Health, Shiraz University of Medical Sciences,
Shiraz, Iran
AUTHOR