TY - JOUR ID - 40372 TI - Effects of Ketamine on Neuronal Spontaneous Excitatory Postsynaptic Currents and Miniature Excitatory Postsynaptic Currents in the Somatosensory Cortex of Rats JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Yuan, Chengdong AU - Zhang, Yajun AU - Zhang, Yu AU - Cao, Song AU - Wang, Yuan AU - Fu, Bao AU - Yu, Tian AD - Guizhou Key Laboratory of Anesthesia and Organ Protection, Zunyi Medical College, Guizhou, China Y1 - 2016 PY - 2016 VL - 41 IS - 4 SP - 275 EP - 282 KW - Ketamine KW - Patch-clamp techniques KW - Excitatory postsynaptic potentials KW - Synaptic transmission DO - N2 - Background: Ketamine is a commonly used intravenous anesthetic which produces dissociation anesthesia, analgesia, and amnesia. The mechanism of ketamine-induced synaptic inhibition in high-level cortical areas is still unknown. We aimed to elucidate the effects of different concentrations of ketamine on the glutamatergic synaptic transmission of the neurons in the primary somatosensory cortex by using the whole-cell patch-clamp method.Methods: Sprague-Dawley rats (11–19 postnatal days, n=36) were used to obtain brain slices (300 μM). Spontaneous excitatory postsynaptic currents (data from 40 neurons) were recorded at a command potential of -70 mV in the presence of bicuculline (a competitive antagonist of GABAA receptors, 30 μM) and strychnine (glycine receptor antagonist, 30 μM). Miniature excitatory postsynaptic currents (data from 40 neurons) were also recorded when 1 μM of tetrodotoxin was added into the artificial cerebrospinal fluid. We used GraphPad Prism5for statistical analysis. Significant differences in the mean amplitude and frequency were tested using the Student paired 2-tailed t test. Values of p UR - https://ijms.sums.ac.ir/article_40372.html L1 - https://ijms.sums.ac.ir/article_40372_d729814251e0209b5e8045c40520f683.pdf ER -