Document Type : Original Article(s)
Authors
1 Oral and Dental Disease Research Center, Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
2 Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Zahedan University of Medical Sciences, Zahedan, Iran
Abstract
Background: Odontogenic cysts and tumors develop from the dental follicle of asymptomatic impacted teeth. Odontogenic tissues express the epidermal growth factor receptor family (EGFR), which mediates cell proliferation, survival, and neoplastic differentiation. The present study aimed to compare the immunohistochemical expression of EGFR and human epidermal growth factor receptor 2 (HER2) in the dental follicle of impacted wisdom teeth with normal and abnormal radiographic size.
Methods: In this analytical study, immunohistochemical staining of EGFR and HER2 was performed on 30 normal and 30 abnormal follicles of impacted third molars. Follicles with a width of <2.5 mm were considered normal, whereas those with a width of ≥2.5 mm were regarded as abnormal. The immunoreactive score (IRS) was used to report the expression levels of EGFR and HER2. The obtained data were analyzed using SPSS software. Age and sex were compared in normal and abnormal groups with independent t test and Chi square test, respectively. P<0.05 was considered statistically significant.
Results: The EGFR and HER2 overall expression was high in all normal and abnormal follicles. The comparison of the percentage of stained cells and intensity of EGFR and HER2 staining in normal and abnormal follicles were not significantly different (P=0.73, P=0.63, P=0.95, respectively).
Conclusion: Due to the high expression of EGFR and HER2 in normal and abnormal follicles, as well as the lack of significant differences in these two groups, the radiographic size of dental follicles might not indicate the potential capabilities of their cells, and more research in this field is recommended.
Keywords
- Lima RL, Holanda-Afonso RC, Moura-Neto V, Bolognese AM, DosSantos MF, Souza MM. Human dental follicle cells express embryonic, mesenchymal and neural stem cells markers. Arch Oral Biol. 2017;73:121-8. doi: 10.1016/j.archoralbio.2016.10.003. PubMed PMID: 27764680.
- Gamal h, Hammuda A, Mohammed S. Detection of Stem Cells in Dental Follicle and Benign Odontogenic Lesions by Immunohistochemical Expression of CD44 and c Myc. Egyptian Dental Journal. 2021;67:2133-42. doi: 10.21608/edj.2021.70335.1570.
- Zhang J, Ding H, Liu X, Sheng Y, Liu X, Jiang C. Dental Follicle Stem Cells: Tissue Engineering and Immunomodulation. Stem Cells Dev. 2019;28:986-94. doi: 10.1089/scd.2019.0012. PubMed PMID: 30968740.
- Haidry N, Singh M, Mamatha NS, Shivhare P, Girish HC, Ranganatha N, et al. Histopathological Evaluation of Dental Follicle Associated with Radiographically Normal Impacted Mandibular Third Molars. Ann Maxillofac Surg. 2018;8:259-64. doi: 10.4103/ams.ams_215_18. PubMed PMID: 30693242; PubMed Central PMCID: PMCPMC6327824.
- Dongol A, Sagtani A, Jaisani MR, Singh A, Shrestha A, Pradhan A, et al. Dentigerous Cystic Changes in the Follicles Associated with Radiographically Normal Impacted Mandibular Third Molars. Int J Dent. 2018;2018:2645878. doi: 10.1155/2018/2645878. PubMed PMID: 29755527; PubMed Central PMCID: PMCPMC5884152.
- Razavi SM, Siadat S, Mehdizadeh M, Movahedian B, Hasheminia D. Immunohistochemical study of correlation between histologic changes and radiologic features in pericoronal tissues of impacted wisdom teeth. J Isfahan Dent Sch. 2012;8:162-71. Persian.
- Bhuyan L, Nishat R, Behura SS, Mahapatra N, Kumar H. Insight into the molecular pathogenesis of odontogenic lesions. J Oral Biosci. 2021;63:35-44. doi: 10.1016/j.job.2020.12.001. PubMed PMID: 33476705.
- Fasano M, Della Corte CM, Viscardi G, Di Liello R, Paragliola F, Sparano F, et al. Head and neck cancer: the role of anti-EGFR agents in the era of immunotherapy. Ther Adv Med Oncol. 2021;13:1758835920949418. doi: 10.1177/1758835920949418. PubMed PMID: 33767760; PubMed Central PMCID: PMCPMC7953226.
- Oprita A, Baloi SC, Staicu GA, Alexandru O, Tache DE, Danoiu S, et al. Updated Insights on EGFR Signaling Pathways in Glioma. Int J Mol Sci. 2021;22. doi: 10.3390/ijms22020587. PubMed PMID: 33435537; PubMed Central PMCID: PMCPMC7827907.
- Lakshminarayana S, Rao RS, Sowmya SV, Augustine D, Patil S, Haragannavar VC. Immunohistochemical analysis of erbb2 in odontogenic lesions: a pilot study. World J Dent. 2021;12:70-3. doi: 10.5005/jp-journals-10015-1796.
- da Silva Baumgart C, da Silva Lauxen I, Filho MS, de Quadros OF. Epidermal growth factor receptor distribution in pericoronal follicles: relationship with the origin of odontogenic cysts and tumors. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2007;103:240-5. doi: 10.1016/j.tripleo.2005.11.009. PubMed PMID: 17234542.
- de Oliveira MG, Lauxen Ida S, Chaves AC, Rados PV, Sant’Ana Filho M. Odontogenic epithelium: immunolabeling of Ki-67, EGFR and survivin in pericoronal follicles, dentigerous cysts and keratocystic odontogenic tumors. Head Neck Pathol. 2011;5:1-7. doi: 10.1007/s12105-010-0216-0. PubMed PMID: 21053110; PubMed Central PMCID: PMCPMC3037468.
- Oikawa M, Miki Y, Shimizu Y, Kumamoto H. Assessment of protein expression and gene status of human epidermal growth factor receptor (HER) family molecules in ameloblastomas. J Oral Pathol Med. 2013;42:424-34. doi: 10.1111/jop.12024. PubMed PMID: 23278525.
- Yang HK, Chen H, Mao F, Xiao QG, Xie RF, Lei T. Downregulation of LRIG2 expression inhibits angiogenesis of glioma via EGFR/VEGF-A pathway. Oncol Lett. 2017;14:4021-8. doi: 10.3892/ol.2017.6671. PubMed PMID: 28943909; PubMed Central PMCID: PMCPMC5605965.
- Mohan BC, Angadi PV. Quantitative and qualitative analysis of epidermal growth factor receptor expression in pericoronal follicles in predicting proliferative potential. Acta Odontol Scand. 2014;72:770-5. doi: 10.3109/00016357.2014.906649. PubMed PMID: 24844785.
- Cimadon N, Lauxen IS, Carrard VC, Sant’Ana Filho M, Rados PV, Oliveira MG. Analysis of the proliferative potential of odontogenic epithelial cells of pericoronal follicles. J Contemp Dent Pract. 2014;15:761-5. doi: 10.5005/jp-journals-10024-1613. PubMed PMID: 25825104.
- Kömerik N, Tufan A, Kapucuoglu N. Comparison of Proliferative Activity of Epithelial Cells between the Follicles of Impacted Teeth. International Journal of Morphology. 2014;32:1296-302. doi: 10.4067/S0717-95022014000400028.
- Saravana GH, Subhashraj K. Cystic changes in dental follicle associated with radiographically normal impacted mandibular third molar. Br J Oral Maxillofac Surg. 2008;46:552-3. doi: 10.1016/j.bjoms.2008.02.008. PubMed PMID: 18406023.
- Satheesan E, Tamgadge S, Tamgadge A, Bhalerao S, Periera T. Histopathological and Radiographic Analysis of Dental Follicle of Impacted Teeth Using Modified Gallego’s Stain. J Clin Diagn Res. 2016;10:ZC106-11. doi: 10.7860/JCDR/2016/16707.7838. PubMed PMID: 27437341; PubMed Central PMCID: PMCPMC4948517.
- Razavi SM, Hasheminia D, Mehdizade M, Movahedian B, Keshani F. The relation of pericoronal third molar follicle dimension and bcl-2/ki-67 expression: An immunohistochemical study. Dent Res J (Isfahan). 2012;9:S26-31. doi: 10.4103/1735-3327.107931. PubMed PMID: 23814558; PubMed Central PMCID: PMCPMC3692196.
- Cabbar F, Guler N, Comunoglu N, Sencift K, Cologlu S. Determination of potential cellular proliferation in the odontogenic epithelia of the dental follicle of the asymptomatic impacted third molars. J Oral Maxillofac Surg. 2008;66:2004-11. doi: 10.1016/j.joms.2008.06.011. PubMed PMID: 18848095.
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