TY - JOUR ID - 46768 TI - A Nuclear Magnetic Resonance-Based Metabolomic Study to Identify Metabolite Differences between Iranian Isolates of Leishmania major and Leishmania tropica JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Tabrizi, Fatemeh AU - Seyyed Tabaei, Seyyed Javad AU - Ahmadi, Nayeb Ali AU - Arefi Oskouie, Afsaneh AD - Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Proteomics Research Center, School of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran AD - Proteomics Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran Y1 - 2021 PY - 2021 VL - 46 IS - 1 SP - 43 EP - 51 KW - Leishmaniasis KW - Leishmania tropica KW - Leishmania Major KW - Metabolomics KW - Proton magnetic resonance spectroscopy DO - 10.30476/ijms.2019.82120.0 N2 - Background: Cutaneous leishmaniasis caused by Leishmania species (L. spp) is one of the most important parasitic diseases in humans. To gain information on the metabolite variations and biochemical pathways between L. spp, we used the comparative metabolome of metacyclic promastigotes in the Iranian isolates of L. major and L. tropica by proton nuclear magnetic resonance (1H-NMR).Methods: L. tropica and L. major were collected from three areas of Iran, namely Gonbad, Mashhad, and Bam, between 2017 and 2018, and were cultured. The metacyclic promastigote of each species was separated, and cell metabolites were extracted. 1H-NMR spectroscopy was applied, and the data were processed using ProMatab in MATLAB (version 7.8.0.347). Multivariate statistical analyses, including the principal component analysis and the orthogonal projections to latent structures discriminant analysis, were performed to identify the discriminative metabolites between the two L. spp. Metabolites with variable influences in projection values of more than one and a P value of less than 0.05 were marked as significant differences. Results: A set of metabolites were detected, and 24 significantly differentially expressed metabolites were found between the metacyclic forms of L. major and L. tropica isolates. The top differential metabolites were methionine, aspartate, betaine, and acetylcarnitine, which were increased more in L. tropica than L. major (p <0.005), whereas asparagine, 3-hydroxybutyrate, L-proline, and kynurenine were increased significantly in L. major (p <0.01). The significantly altered metabolites were involved in eight metabolic pathways. Conclusion: Metabolomics, as an invaluable technique, yielded significant metabolites, and their biochemical pathways related to the metacyclic promastigotes of L. major and L. tropica. The findings offer greater insights into parasite biology and how pathogens adapt to their hosts. UR - https://ijms.sums.ac.ir/article_46768.html L1 - https://ijms.sums.ac.ir/article_46768_c7a91a791b9ab854330fe76c4754b74c.pdf ER -