TY - JOUR ID - 45423 TI - Molecular Detection and Serotyping of Streptococcus Pneumoniae in Children with Suspected Meningitis in Northeast Iran JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Abdoli, Sepideh AU - Safamanesh, Saghar AU - Khosrojerdi, Mahsa AU - Azimian, Amir AD - Department of Biotechnology and Molecular Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran AD - Department of Pathobiology and Laboratory Sciences, Faculty of Medicine, North Khorasan University of Medical Sciences, Bojnurd, Iran; AD - Department of Pediatrics, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran Y1 - 2020 PY - 2020 VL - 45 IS - 2 SP - 125 EP - 133 KW - Streptococcus Pneumoniae KW - Serogroup KW - Vaccines KW - Meningitis DO - 10.30476/ijms.2019.45423 N2 - Background: To date, more than 90 Streptococcus pneumoniae (S. pneumoniae) capsular serotypes are known. The prevalence of these serotypes varies according to the geographical area and the regional vaccination program. Due to the lack of regular vaccination programs for S. pneumoniae in developing countries, serotyping of the prevalent isolates is useful in selecting the correct vaccine. The present study aimed to evaluate common serotypes of pneumococcal meningitis in Bojnurd, Iran.Methods: All cerebrospinal fluid (CFS) samples suspected for bacterial meningitis were analyzed. The samples were collected during 2014-2018 in the Laboratory of Imam Reza Hospital (Bojnurd, Iran). Due to the high rate of false-negative cultures, polymerase chain reaction (PCR) was used for the detection of lytA and psaA genes of S. pneumoniae. In addition, the modified Marimon’s PCR method was used for serotyping the bacteria. The data were analyzed using Pearson’s Chi-square test. Results: Out of the 901 CSF samples, 106 cases tested positive for S. pneumoniae using the PCR method, while only 92 cases tested positive using the conventional methods. Based on the Marimon’s PCR method, serotypes 23F, 19F, 19A, 1, 14, and serogroup 6A/B were the most common types. Serogroups 18C, 15A/F, 15B/C, 9A/V, 7A/F, 11A/D/F, and 22A/F were also detected in isolates. Note that 2.8% of the samples were non-typable (NT).Conclusion: The results showed that only 13 serotypes were responsible for all meningitis cases. Pneumococcal capsular vaccine-13 (PCV-13) is the preferred choice against common serotypes of S. pneumoniae in northeast Iran. The abstract was presented in Iran’s 19th International Congress of Microbiology, as a poster and published in the congress abstracts book. UR - https://ijms.sums.ac.ir/article_45423.html L1 - https://ijms.sums.ac.ir/article_45423_683880989dc9491e05c9c07ce9ce4ec9.pdf ER -