@article { author = {Ghanbari, Hamideh and Keshtgar, Sara and Kazeroni, Marjaneh}, title = {Inhibition of the CatSper Channel and NOX5 Enzyme Activity Affects the Functions of the Progesterone-Stimulated Human Sperm}, journal = {Iranian Journal of Medical Sciences}, volume = {43}, number = {1}, pages = {18-25}, year = {2018}, publisher = {Shiraz University of Medical Sciences}, issn = {0253-0716}, eissn = {1735-3688}, doi = {10.30476/ijms.2018.40505}, abstract = {Background: Low levels of reactive oxygen species (ROS) and calcium are necessary for sperm function. NADPH oxidase 5 (NOX5) is a membrane enzyme which produces ROS. This enzyme is dependent on calcium for its activity. We investigated the importance of NOX5 and an important calcium channel (CatSper) on sperm function.Methods: This laboratory in-vitro study was done in Shiraz, Iran, 2016. Normal semen samples (n=24) were washed and diluted to 20×106 sperm/mL. The diluted samples were divided into 8 groups, containing Ham’s F-10 (control group), 2 µM of NNC (CatSper channel inhibitor), 1 µM DPI (NOX5 inhibitor), and NNC+DPI. The other 4 groups were the same as the 1st ones, except that they contained 1 µM of progesterone. Motility assessment was done by VT–Sperm 3.1. Acrosome status was monitored with acrosome-specific FITC-PSA using fluorescent microscopy. Sperm viability was assessed by Eosin Y. Statistical analysis was performed using SPSS 16 software. The comparison between the groups was done using the one-way ANOVA, followed by Tukey. A P}, keywords = {NOX5 protein,Human,CatSper,Progesterone,Sperm,Acrosome reaction}, url = {https://ijms.sums.ac.ir/article_40505.html}, eprint = {https://ijms.sums.ac.ir/article_40505_46d93a83f4a4a64c1839b13432a26e5d.pdf} }