TY - JOUR ID - 45772 TI - The Effect of Bifidobacterium Bifidum Supernatant and Cell Mass on the Proliferation Potential of Rat Bone Marrow-Derived Stromal Cells JO - Iranian Journal of Medical Sciences JA - IJMS LA - en SN - 0253-0716 AU - Saberian, Mostafa AU - Shahidi Delshad, Elham AU - Habibi, Masoud AD - Department of Cellular and Molecular Nutrition, School of Nutritional Sciences and Dietetics, Tehran University of Medical Sciences. Tehran, Iran AD - Shahid Rajaei Cardiovascular Research Center, Iran University of Medical Sciences, Tehran, Iran AD - Department of Genetic, Breast Cancer Research Center, Motamed Cancer Institute, ACECR, Tehran, Iran Y1 - 2020 PY - 2020 VL - 45 IS - 4 SP - 269 EP - 276 KW - Bifidobacterium bifidum KW - Mesenchymal stromal cells KW - Cell Proliferation KW - Probiotics DO - 10.30476/ijms.2019.45772 N2 - Background: Mesenchymal stem cells (MSCs) are widely used to treat various diseases, however, their proliferative potential reduces after a number of passages. It has been shown that some probiotics such as Bifidobacterium bifidum (B. bifidum) affect the proliferation of various cell lineages. The present study aimed to investigate the effect of B. bifidum on the proliferation of rat bone marrow stromal cells (rBMSCs) and to develop a method for compensating their proliferation reduction after some passages. Methods: The present experimental study was conducted at Tehran University of Medical Sciences, Tehran, Iran, in 2017. The stromal cells were isolated from rBMSCs and their mesenchymal properties were confirmed by osteogenic and adipogenic differentiation media and staining. B. bifidum was cultured and the B. bifidum supernatant (BS) and bacterial cell mass (BCM) were extracted. The rBMSCs were treated with different concentrations of BS and BCM. The MTT assay was performed to measure the number of viable cells in the culture. Cell proliferation was analyzed using the paired-sample t test.Results: Cell proliferation increased as the concentration of bacteria was increased logarithmically (0, 0.1, 0.3, 0.9, 3, 9, 30 μL/mL). In comparison with BS, cells treated with BCM showed increased cell proliferation at lower concentrations. This effect was caused by removing the “de Man, Rogosa, and Sharpe” (MRS) broth medium from the BCM culture. The optimal concentration of bacteria with the most significant effect on rBMSCs proliferation was determined.Conclusion: A significant increase in the proliferation of stromal cells was observed; confirming the stimulatory potential of probiotics (B. bifidum) on various cells. The use of products containing probiotic bacteria can increase the proliferation potential of BMSCs. UR - https://ijms.sums.ac.ir/article_45772.html L1 - https://ijms.sums.ac.ir/article_45772_d7b9aa819cc4f63a6d7f63b1a7b43bc6.pdf ER -