Document Type: Original Article(s)
Department of Oral and Maxillofacial Surgery, School of Dentistry, Shiraz University of Medical Sciences, Shiraz Iran
Department of Tissue Engineering, National Institute of Genetic Engineering and Biotechnology, Iran
Stem Cell Lab, Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Tissue Engineering Lab, Department
of Anatomy, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Background: Platelet-rich plasma (PRP) and bioceramics such as hydroxyapatite (HA) and zirconium oxide (ZrO2) are used to reconstruct mandibular defects. We sought to determine the synergistic effects of HA/ZrO2 and PRP and compare their osteogenic activity.Methods: ZrO2 scaffolds were constructed by the slurry method and were then coated with HA and impregnated by PRP/heparan sulfate (HS). Bilateral mandibular defects were created in 26 male rabbits. In 20 rabbits, the left defects were treated with HA/ZrO2/PRP (Group 1) and the corresponding right defects were filled with HA/ZrO2 (Group 2). The 6 remaining models were treated with PRP gels at both sides (Group 3). The osteoconductivity of HA/ZrO2/PRP was compared with that of HA/ZrO2 or PRP by radiological and histological methods after the follow-up period, at weeks 6, 8, and 12. The statistical analyses were performed by ANOVA and LSD using SPSS, version 16.0, for Windows (P<0.05).Results: After 2 weeks, the percentage of the surface occupied by bone was significantly higher in the HA/ZrO2/PRP-treated defects than in the PRP-treated defects (P=0.007). Osteoblast and osteocyte counts were higher significantly in the PRP-treated group (P=0.032); however, the cells had not started matrix formation on a large scale and just small islands of osteoid with trapped osteocytes were observed. In the long term, the regenerative potential of all the scaffolds was the same. Conclusion: HA/ZrO2 showed a superior osteoconductive capacity over PRP in the short term; however, they showed no long-term synergic effects.