Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

Comparison of Cytotoxic Activity of Bile on HepG2 and CCRF-CEM Cell Lines: An in Vitro Study

Document Type : Brief Report(s)

Authors

Negar Azarpira
Fatemeh Rastegar
Maryam Amiri
Elaheh Esfandiari
Bita Geramizadeh

Organ Transplant Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract
The aim of this study was to examine the effect of crude bile on the human HepG2 and CCRF-CEM cell lines. Cells were exposed to different dilutions of bile. Antiproliferative effects were determined by the cytotoxic MTT assay. Cells undergoing apoptosis were identified by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay. Bile administration resulted in dose-dependent cytotoxicity in both HepG2 and CCRF-CEM cell lines. Incubated cells exhibited morphologic features of apoptosis. Bile has significant cytotoxic activity in HepG2 and CCRF-CEM cancer cells via induction of apoptosis. The mechanism of apoptosis needs to be further evaluated. It may have clinical utility in the treatment of cancer after in vivo confirmation of activity.

Keywords

Bile
Neoplasms
Cytotoxicity
Iranian Journal of Medical Sciences
Volume 37, Issue 4 - Serial Number 4
10
December 2012
Pages 266-270

  • Received 04 December 2013
  • Revised 24 August 2020
  • Accepted 24 December 2018

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