Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

Evaluation of a PCR Assay to Detect Enterococcus faecalis in Blood and Determine Glycopeptides Resistance Genes: Van A and Van B

Document Type : Original Article(s)

Authors

Hamidreza Honarmand 1
Mahsome Falah Ghavidel 2
Iraj Nikokar 3
Morteza Rahbar Taromsari 4

1 Cellular and Molecular Research Center, Guilan University of Medical Sciences, Guilan, Iran

2 Department of Microbiology, Azad University, Lahijan, Iran

3 Department of Microbiology, School of Paramedicine, Guilan University of Medical Sciences, Guilan, Iran

4 Department of Legal Medicine, Razi Hospital, Guilan University of Medical Sciences, Guilan, Iran

Abstract
Background: Bacteremia due to Enterococcus faecalis is usually caused by strains resistant to most antibiotics. Effective management of the disease is dependent on rapid detection and characterization of the bacteria, and determination its sensitivity pattern to antimicrobial drugs. The aim of this study was to investigate a more rapid and reliable assay for simultaneous diagnosis of enterococcal bacteremia and its sensitivity pattern to antimicrobial drugs.
Methods: Several bacterial suspensions with different content of two standard strains of Enterococcus faecalis resistant to vancomycin were used for inoculation to defibrinated sheep blood samples. PCR and routine assay was performed on all blood samples with different bacterial content.
Results: Routine assay and PCR for all inoculated blood samples with ≥5 cfu/ml was positive. Mean time for PCR and routine assays was 10 hours and 5 days, respectively.
Conclusion: PCR is a more rapid and sensitive assay for simultaneous detection and characterization for Enterococcus faecalis, and determination of its sensitivity pattern to vancomycin.

Keywords

Enterococcus faecalis
multiplex-PCR
Van A
Van B
Iranian Journal of Medical Sciences
Volume 37, Issue 3 - Serial Number 3
11
September 2012
Pages 194-199

  • Received 08 December 2013
  • Revised 24 August 2020
  • Accepted 24 December 2018

Home

Guide for Authors

Submit Manuscript

Guide for Reviewers

Forms

News

Contact Us

FAQ

Suggestion and Complain