Document Type: Brief Report(s)
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
Aspirin is an anti-inflammatory drug, peroxyl radical scavenger, and antioxidant agent that inhibits phospholipases, nitric oxide synthetases, and cyclooxygenase enzymes. The existing literature contains no studies on the effects of various doses of aspirin on spinal cord injury (SCI). Therefore, we sought to investigate the putative effects of aspirin on experimental SCI.The weight-drop injury model was used to produce SCI in 100 albino Wistar rats. The animals were allocated to five groups: a control group, where the rats did not undergo any surgical or medical intervention except for anesthesia; a sham-treated group, where laminectomy was performed without SCI and no further therapy was administered; and three other groups, where the rats with SCI received low-dose aspirin [20 mg/kg], high-dose aspirin [80 mg/kg], and a vehicle, respectively. Half of the rats were sacrificed 24 hours later, and their spinal cords were excised for biochemical studies. The other rats were subjected to Basso, Beattie, and Bresnahan (BBB) locomotor rating scale scoring once a week for 6 consecutive weeks.Aspirin decreased lipid peroxidation following SCI as the mean (± standard error) catalase level was significantly higher in the high-dose aspirin group (46.10±12.01) than in the sham-treated group (16.07±2.42) and the vehicle-treated group (15.31±3.20) (P<0.05; P<0.05, respectively). Both of the groups treated with high-dose and low-dose aspirin demonstrated a higher mean BBB score than did the control group (P<0.001) and the sham-treated group (P<0.001).Our data provide evidence in support of the potential effects of aspirin in biochemical and neurobehavioral recovery after SCI
- van den Berg ME, Castellote JM, Mahillo-Fernandez I, de Pedro-Cuesta J. Incidence of spinal cord injury worldwide: a systematic review. Neuroepidemiology. 2010;34:184-92; discussion 92. doi: 10.1159/000279335. PubMed PMID: 20130419.
- Yip PK, Malaspina A. Spinal cord trauma and the molecular point of no return. Mol Neurodegener. 2012;7:6. doi: 10.1186/1750-1326-7-6. PubMed PMID: 22315999; PubMed Central PMCID: PMC3299607.
- Saunders RD, Dugan LL, Demediuk P, Means ED, Horrocks LA, Anderson DK. Effects of methylprednisolone and the combination of alpha-tocopherol and selenium on arachidonic acid metabolism and lipid peroxidation in traumatized spinal cord tissue. J Neurochem. 1987;49:24-31. PubMed PMID: 3108455.
- Basso DM, Beattie MS, Bresnahan JC. A sensitive and reliable locomotor rating scale for open field testing in rats. J Neurotrauma. 1995;12:1-21. doi: 10.1089/neu.1995.12.1. PubMed PMID: 7783230.
- Erol FS, Kaplan M, Tiftikci M, Yakar H, Ozercan I, Ilhan N, et al. Comparison of the effects of octreotide and melatonin in preventing nerve injury in rats with experimental spinal cord injury. J Clin Neurosci. 2008;15:784-90. doi: 10.1016/j.jocn.2007.06.009. PubMed PMID: 18407497.
- Pantovic R, Draganic P, Erakovic V, Blagovic B, Milin C, Simonic A. Effect of indomethacin on motor activity and spinal cord free fatty acid content after experimental spinal cord injury in rabbits. Spinal Cord. 2005;43:519-26. doi: 10.1038/sj.sc.3101763. PubMed PMID: 15852057.
- Yeo JE, Kim JH, Kang SK. Selenium attenuates ROS-mediated apoptotic cell death of injured spinal cord through prevention of mitochondria dysfunction; in vitro and in vivo study. Cell Physiol Biochem. 2008;21:225-38. doi: 10.1159/000113764. PubMed PMID: 18209489.
- Ates O, Cayli S, Altinoz E, Gurses I, Yucel N, Kocak A, et al. Effects of resveratrol and methylprednisolone on biochemical, neurobehavioral and histopathological recovery after experimental spinal cord injury. Acta Pharmacol Sin. 2006;27:1317-25. doi: 10.1111/j.1745-7254.2006.00416.x. PubMed PMID: 17007738.
- Gok B, Okutan O, Beskonakli E, Kilinc K. Effects of magnesium sulphate following spinal cord injury in rats. Chin J Physiol. 2007;50:93-7. PubMed PMID: 17608147.
- Kaptanoglu E, Solaroglu I, Okutan O, Surucu HS, Akbiyik F, Beskonakli E. Erythropoietin exerts neuroprotection after acute spinal cord injury in rats: effect on lipid peroxidation and early ultrastructural findings. Neurosurg Rev. 2004;27:113-20. doi: 10.1007/s10143-003-0300-y. PubMed PMID: 12920606.
- Fujita Y, Shingu T, Kurihara M, Miyake H, Kono T, Tsujimura M, et al. Evaluation of a low dose administration of aspirin, dipyridamol and steroid. Therapeutic effects on motor function and protective effects on Na+-K+-activated ATPase activity against lipid peroxidation in an experimental model of spinal cord injury. Paraplegia. 1985;23:56-7. doi: 10.1038/sc.1985.9. PubMed PMID: 2984632.
- Kwon BK, Okon E, Hillyer J, Mann C, Baptiste D, Weaver LC, et al. A systematic review of non-invasive pharmacologic neuroprotective treatments for acute spinal cord injury. J Neurotrauma. 2011;28:1545-88. doi: 10.1089/neu.2009.1149. PubMed PMID: 20146558; PubMed Central PMCID: PMC3143410.
- Charan J, Kantharia N. How to calculate sample size in animal studies? Journal of Pharmacology & Pharmacotherapeutics. 2013;4:303. doi: 10.4103/0976-500X.119726.
- Metz GA, Curt A, van de Meent H, Klusman I, Schwab ME, Dietz V. Validation of the weight-drop contusion model in rats: a comparative study of human spinal cord injury. J Neurotrauma. 2000;17:1-17. 10.1089/neu.2000.17.1. PubMed PMID: 10674754.
- Dogruer ZN, Unal M, Eskandari G, Pata YS, Akbas Y, Cevik T, et al. Malondialdehyde and antioxidant enzymes in children with obstructive adenotonsillar hypertrophy. Clin Biochem. 2004;37:718-21. doi: 10.1016/j.clinbiochem.2004.01.004. PubMed PMID: 15302619.
- Berker KI, Ozdemir Olgun FA, Ozyurt D, Demirata B, Apak R. Modified Folin-Ciocalteu antioxidant capacity assay for measuring lipophilic antioxidants. J Agric Food Chem. 2013;61:4783-91. doi: 10.1021/jf400249k. PubMed PMID: 23627440.
- Rangan U, Bulkley GB. Prospects for treatment of free radical-mediated tissue injury. Br Med Bull. 1993;49:700-18. PubMed PMID: 8221033.
- Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5:9-19. doi: 10.1097/WOX.0b013e3182439613. PubMed PMID: 23268465; PubMed Central PMCID: PMC3488923.
- Grosser N, Schroder H. Aspirin protects endothelial cells from oxidant damage via the nitric oxide-cGMP pathway. Arterioscler Thromb Vasc Biol. 2003;23:1345-51. doi: 10.1161/01.ATV.0000083296.57581.AE. PubMed PMID: 12829521.
- He Z, Liao Q, Xu X, Luo C, Zhou T, Li Q, et al. A study of the acting mechanism of aspirin for resistance to oxidative damage. Hua Xi Yi Ke Da Xue Xue Bao. 2001;32:413-6. PubMed PMID: 12536580.