Document Type : Original Article(s)
Authors
- Maryam Heidari 1
- Naghmeh Sattarahmady 2
- Sirus Javadpour 3
- Negar Azarpira 4
- Hossein Heli 5
- Alireza Mehdizadeh 2
- Amirhossein Rajaei 6
- Tahereh Zare 1
1 Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
2 Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran; and Nanomedicine and Nanobiology Research Center, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
3 Department of Materials Science and Engineering, School of Electrical and Computer Engineering, Shiraz University, Shiraz, Iran
4 Transplant Research Center, Department of Pathology, Namazee Teaching Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Nanomedicine, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran
6 Department of Electrical and Electronic, School of Electrical and Electronic Engineering, Shiraz University of Technology, Shiraz, Iran
Abstract
Background: Nowadays, magnetic nanoparticles (MNPs) have received much attention because of their enormous potentials in many fields such as magnetic fluid hyperthermia (MFH). The goal of hyperthermia is to increase the temperature of malignant cells to destroy them without any lethal effect on normal tissues. To investigate the effectiveness of cancer therapy by magnetic fluid hyperthermia, Fe0.5Zn0.5Fe2O4 nanoparticles (FNPs) were used to undergo external magnetic field (f=515 kHz, H=100 G) in mice bearing implanted tumor.Methods: FNPs were synthesized via precipitation and characterized using transmission electron microscopy (TEM), vibrating sample magnetometer, and Fourier transform infrared. For in vivo study, the mice bearing implanted tumor were divided into four groups (two mice per group), namely, control group, AMF group, MNPs group, and MNPs&AMF group. After 24 hours, the mice were sacrificed and each tumor specimen was prepared for histological analyses. The necrotic surface area was estimated by using graticule (Olympus, Japan) on tumor slides.Results: The mean diameter of FNPs was estimated around 9 nm by TEM image and M versus H curve indicates that this particle is among superparamagnetic materials. According to histological analyses, no significant difference in necrosis extent was observed among the four groups.Conclusion: FNPs are biocompatible and have a good size for biomedical applications. However, for MFH approach, larger diameters especially in the range of ferromagnetic particles due to hysteresis loss can induce efficient heat in the target region.
Keywords
- Doaga A, Cojocariu A, Amin W, Heib F, Bender P, Hempelmann R, et al. Synthesis and characterizations of manganese ferrites for hyperthermia applications. Mater Chem Phys. 2013;143:305-10. doi: 10.1016/j.matchemphys.2013.08.066.
- Kozissnik B, Bohorquez AC, Dobson J, Rinaldi C. Magnetic fluid hyperthermia: advances, challenges, and opportunity. Int J Hyperthermia. 2013;29:706-14. doi: 10.3109/02656736.2013.837200. PubMed PMID: 24106927.
- Sun H, Xu L, Fan T, Zhan H, Wang X, Zhou Y, et al. Targeted hyperthermia after selective embolization with ferromagnetic nanoparticles in a VX2 rabbit liver tumor model. Int J Nanomedicine. 2013;8:3795-804. doi: 10.2147/IJN.S50373. PubMed PMID: 24124367; PubMed Central PMCID: PMC3795007.
- Kut C, Zhang Y, Hedayati M, Zhou H, Cornejo C, Bordelon D, et al. Preliminary study of injury from heating systemically delivered, nontargeted dextran-superparamagnetic iron oxide nanoparticles in mice. Nanomedicine (Lond). 2012;7:1697-711. doi: 10.2217/nnm.12.65. PubMed PMID: 22830502; PubMed Central PMCID: PMC3991127.
- Motoyama J, Yamashita N, Morino T, Tanaka M, Kobayashi T, Honda H. Hyperthermic treatment of DMBA-induced rat mammary cancer using magnetic nanoparticles. Biomagn Res Technol. 2008;6:2. doi: 10.1186/1477-044X-6-2. PubMed PMID: 18298831; PubMed Central PMCID: PMC2266920.
- Bakoglidis K, Simeonidis K, Sakellari D, Stefanou G, Angelakeris M. Size-dependent mechanisms in AC magnetic hyperthermia response of iron-oxide nanoparticles. IEEE Trans Magn. 2012;48:1320-3. doi: 10.1109/TMAG.2011.2173474.
- Kumar CS, Mohammad F. Magnetic nanomaterials for hyperthermia-based therapy and controlled drug delivery. Adv Drug Deliv Rev. 2011;63:789-808. doi: 10.1016/j.addr.2011.03.008. PubMed PMID: 21447363; PubMed Central PMCID: PMC3138885.
- Di Barba P, Dughiero F, Sieni E. Magnetic field synthesis in the design of inductors for magnetic fluid hyperthermia. IEEE Trans Magn. 2010;46:2931-4. doi: 10.1109/TMAG.2010.2044769.
- Bettge M, Chatterjee J, Haik Y. Physically synthesized Ni-Cu nanoparticles for magnetic hyperthermia. Biomagn Res Technol. 2004;2:4. doi: 10.1186/1477-044X-2-4. PubMed PMID: 15132747; PubMed Central PMCID: PMC420488.
- Pradhan P, Giri J, Rieken F, Koch C, Mykhaylyk O, Doblinger M, et al. Targeted temperature sensitive magnetic liposomes for thermo-chemotherapy. J Control Release. 2010;142:108-21. doi: 10.1016/j.jconrel.2009.10.002. PubMed PMID: 19819275.
- Pradhan P, Giri J, Samanta G, Sarma HD, Mishra KP, Bellare J, et al. Comparative evaluation of heating ability and biocompatibility of different ferrite-based magnetic fluids for hyperthermia application. J Biomed Mater Res B Appl Biomater. 2007;81:12-22. doi: 10.1002/jbm.b.30630. PubMed PMID: 16924619.
- Balivada S, Rachakatla RS, Wang H, Samarakoon TN, Dani RK, Pyle M, et al. A/C magnetic hyperthermia of melanoma mediated by iron(0)/iron oxide core/shell magnetic nanoparticles: a mouse study. BMC Cancer. 2010;10:119. doi: 10.1186/1471-2407-10-119. PubMed PMID: 20350328; PubMed Central PMCID: PMC2859385.
- Zhao Q, Wang L, Cheng R, Mao L, Arnold RD, Howerth EW, et al. Magnetic nanoparticle-based hyperthermia for head & neck cancer in mouse models. Theranostics. 2012;2:113-21. doi: 10.7150/thno.3854. PubMed PMID: 22287991; PubMed Central PMCID: PMC3267386.
- KöseoÄlu Y, Bay M, Tan M, Baykal A, Sözeri H, Topkaya R, et al. Magnetic and dielectric properties of Mn0. 2Ni0. 8Fe2O4 nanoparticles synthesized by PEG-assisted hydrothermal method. J Nanopart Res. 2011;13:2235-44.
- Hankare P, Patil R, Sankpal U, Jadhav S, Lokhande P, Jadhav K, et al. Investigation of structural and magnetic properties of nanocrystalline manganese substituted lithium ferrites. J Solid State Chem. 2009;182:3217-21. doi: 10.1016/j.jssc.2009.08.034.
- Varshosaz J, Sadeghi-aliabadi H, Ghasemi S, Behdadfar B. Use of magnetic folate-dextran-retinoic acid micelles for dual targeting of doxorubicin in breast cancer. Biomed Res Int. 2013;2013:680712. doi: 10.1155/2013/680712. PubMed PMID: 24381941; PubMed Central PMCID: PMC3870081.
- Hong R, Feng B, Chen L, Liu G, Li H, Zheng Y, et al. Synthesis, characterization and MRI application of dextran-coated Fe 3 O 4 magnetic nanoparticles. Biochem Eng J. 2008;42:290-300.
- Hong R, Li J, Qu J, Chen L, Li H. Preparation and characterization of magnetite/dextran nanocomposite used as a precursor of magnetic fluid. Chem Eng J. 2009;150:572-80. doi: 10.1016/j.cej.2009.03.034.
- Kolhatkar AG, Jamison AC, Litvinov D, Willson RC, Lee TR. Tuning the magnetic properties of nanoparticles. Int J Mol Sci. 2013;14:15977-6009. doi: 10.3390/ijms140815977. PubMed PMID: 23912237; PubMed Central PMCID: PMC3759896.
- Tiefenauer LX, Kuhne G, Andres RY. Antibody-magnetite nanoparticles: in vitro characterization of a potential tumor-specific contrast agent for magnetic resonance imaging. Bioconjug Chem. 1993;4:347-52. doi: 10.1021/bc00023a007. PubMed PMID: 8274518.
- Eberbeck D, Lange A, Hentschel M. Identification of aggregates of magnetic nanoparticles in ferrofluids at low concentrations. J Appl Crystallogr. 2003;36:1069-74. doi: 10.1107/S002188980301104X.
- Morales M, Bomati-Miguel O, De Alejo RP, Ruiz-Cabello J, Veintemillas-Verdaguer S, OâGrady K. Contrast agents for MRI based on iron oxide nanoparticles prepared by laser pyrolysis. Journal of Magnetism and Magnetic Materials. 2003;266:102-9. doi: 10.1016/S0304-8853(03)00461-X.
- Abdeen S, Praseetha P. Diagnostics and treatment of metastatic cancers with magnetic nanoparticles. J Nanomedine Biotherapeutic Discov. 2013;3:115. doi: 10.4172/2155-983X.1000115.
)