Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

Inhibition of the CatSper Channel and NOX5 Enzyme Activity Affects the Functions of the Progesterone-Stimulated Human Sperm

Document Type : Original Article(s)

Authors

Hamideh Ghanbari 1
Sara Keshtgar 1
Marjaneh Kazeroni 2

1 Department of Physiology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

2 Shiraz Infertility Center, Shiraz, Iran

https://doi.org/10.30476/ijms.2018.40505
Abstract
Background: Low levels of reactive oxygen species (ROS) and calcium are necessary for sperm function. NADPH oxidase 5 (NOX5) is a membrane enzyme which produces ROS. This enzyme is dependent on calcium for its activity. We investigated the importance of NOX5 and an important calcium channel (CatSper) on sperm function.Methods: This laboratory in-vitro study was done in Shiraz, Iran, 2016. Normal semen samples (n=24) were washed and diluted to 20×106 sperm/mL. The diluted samples were divided into 8 groups, containing Ham’s F-10 (control group), 2 µM of NNC (CatSper channel inhibitor), 1 µM DPI (NOX5 inhibitor), and NNC+DPI. The other 4 groups were the same as the 1st ones, except that they contained 1 µM of progesterone. Motility assessment was done by VT–Sperm 3.1. Acrosome status was monitored with acrosome-specific FITC-PSA using fluorescent microscopy. Sperm viability was assessed by Eosin Y. Statistical analysis was performed using SPSS 16 software. The comparison between the groups was done using the one-way ANOVA, followed by Tukey. A P

Keywords

NOX5 protein
Human
CatSper
Progesterone
Sperm
Acrosome reaction
Iranian Journal of Medical Sciences
Volume 43, Issue 1 - Serial Number 1
91
January 2018
Pages 18-25


  • Received 09 October 2016
  • Revised 16 March 2020
  • Accepted 24 December 2018

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