Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

Hyperuricemia Induces Wnt5a/Ror2 Gene Expression, Epithelial–Mesenchymal Transition, and Kidney Tubular Injury in Mice

Document Type : Original Article(s)

Authors

Wiwit Ananda Wahyu Setyaningsih 1
Nur Arfian 2
Efrayim Suryadi
Muhammad Mansyur Romi
Untung Tranggono
Dwi Cahyani Ratna Sari

1 Department of Anatomy Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia

2 Department of Anatomy Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Pharmaco Street, North Sekip, Yogyakarta 55281, Indonesia

https://doi.org/10.30476/ijms.2018.40525
Abstract
Background: Hyperuricemia contributes to kidney injury, characterized by tubular injury with epithelial–mesenchymal transition (EMT). Wnt5a/Ror2 signaling drives EMT in many kidney pathologies. This study sought to evaluate the involvement of Wnt5a/Ror2 in hyperuricemia-induced EMT in kidney tubular injury.Methods: A hyperuricemia model was performed in male Swiss background mice (3 months old, 30–40 g) with daily intraperitoneal injections of 125 mg/kg body weight (BW) of uric acid. The mice were terminated on day 7 (UA7, n=5) and on day 14 (UA14, n=5). Allopurinol groups (UAl7 and UAl14, each n=5) were added with oral 50 mg/kg BW of allopurinol treatment. The serum uric acid level was quantified, and tubular injury was assessed based on PAS staining. Reverse transcriptase-PCR was done to quantify Wnt5a, Ror2, E-cadherin, and vimentin expressions. IHC staining was done for E-cadherin and collagen I. We used the Shapiro–Wilk for normality testing and one-way ANOVA for variance analysis with a P

Keywords

Hyperuricemia
Tubular injury
E-cadherin gene expression
Vimentin
Gene expression
Wnt5a/Ror2
Iranian Journal of Medical Sciences
Volume 43, Issue 2 - Serial Number 2
92
March 2018
Pages 164-173


  • Received 04 November 2016
  • Revised 16 March 2020
  • Accepted 24 December 2018

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