Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

Effects of Two-by-Two Combination Therapy with Valproic Acid, Lithium Chloride, and Celecoxib on the Angiogenesis of the Chicken Chorioallantoic Membrane

Document Type : Original Article(s)

Authors

Ehsan Afzal 1
Sedigheh Alinezhad 1
Marjan Khorsand 1
Mohammad Javad Khoshnood 2
Mohammad Ali Takhshid

1 Diagnostic Laboratory Sciences and Technology Research Center, Paramedical School, Shiraz University of Medical Sciences, Shiraz, Iran

2 School of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran

https://doi.org/10.30476/ijms.2018.40571
Abstract
Background: The synergistic effects of valproic acid (VPA), lithium (Li), and celecoxib (CX) have been shown in combination therapy against the proliferation and metastasis of numerous cancers. Angiogenesis plays a critical role in the pathogenesis of tumor growth and metastasis. The aim of the present study was to evaluate the antiangiogenic effects of VPA, lithium chloride (LiCl), and CX, alone or in 2-by-2 combinations, using the chicken chorioallantoic membrane (CAM) assay.Methods: Fertilized chicken eggs were randomly divided into 10 groups: control, VPA (1.8 and 3.6 µmol/CAM), Li (0.15 and 0.60 µmol/CAM), CX (0.02 and 0.08 µmol/CAM), VPA+Li, VPA+CX, and CX+Li (n=10 per group). A window was made on the eggshells and the CAMs were exposed to a filter disk containing VPA, LiCl, and CX, alone or in 2-by-2 combinations. The control CAMs were treated with distilled water (vehicle). Three days after the treatment, the number of vessel branch points was counted in each CAM. The data were analyzed using SPSS, version 15.One-way ANOVA, followed by the Tukey tests, was used to compare the groups. A P

Keywords

Angiogenesis inhibitors
Combined modality therapy
Valproic acid
Celecoxib
Lithium chloride
Iranian Journal of Medical Sciences
Volume 43, Issue 5 - Serial Number 5
95
September 2018
Pages 506-513


  • Received 04 March 2017
  • Revised 16 March 2020
  • Accepted 24 December 2018

Home

Guide for Authors

Submit Manuscript

Guide for Reviewers

Forms

News

Contact Us

FAQ

Suggestion and Complain