Document Type : Original Article(s)
Authors
- Sayed Mohammad Shafiee 1
- Atefeh Seghatoleslam 1
- Mohsen Nikseresht 2
- Seyed Vahid Hosseini 3
- Mahvash Alizadeh-Naeeni 4
- Akbar Safaei 5
- Ali Akbar Owji 6
1 Department of Biochemistry-Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2 Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran
3 Colorectal Research Center, Shahid Faghihi Hospital, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
4 Department of Internal Medicine, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5 Department of Pathology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
6 Department of Biochemistry-Recombinant Protein Laboratory, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran, and Endocrine and Metabolism Research Center, Nemazee Hospital, Shiraz University of Medical Sciences, Shiraz, Iran
Abstract
Background: Activation of the ubiquitin-proteasome pathway in various malignancies, including colorectal cancer, is established. This pathway mediates the degradation of damaged proteins and regulates growth and stress response. The novel human gene, UBE2Q2, with a putative ubiquitin-conjugating enzyme activity, is reported to be overexpressed in some malignancies. We sought to investigate the expression levels of the UBE2Q2 gene in colorectal cell lines as well as in cancerous and normal tissues from patients with colorectal cancer. Methods: Levels of UBE2Q2 mRNA in cell lines were assessed by Real-Time PCR. Western blotting was employed to investigate the levels of the UBE2Q2 protein in 8 colorectal cell lines and 43 colorectal tumor samples. Results: Expression of UBE2Q2 was observed at the level of both mRNA and protein in colorectal cell lines, HT29/219, LS180, SW742, Caco2, HTC116, SW48, SW480, and SW1116. Increased levels of UBE2Q2 immunoreactivity was observed in the 65.11% (28 out of 43) of the colorectal carcinoma tissues when compared with their corresponding normal tissues. Difference between the mean intensities of UBE2Q2 bands from cancerous and normal tissues was statistically significant at P
Keywords
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