Document Type : Original Article(s)
Authors
- Fatemeh Akbari 1
- Seyed Jalal Hosseinimehr 2
- Marzieh Noruz 1
- Fatemeh Ebrahimi 2
- Ali Bagheri 3
- Lale Vahedi 4
- Mohammad Azadbakht 5
1 Department of Pharmacognosy, School of Pharmacy, Semnan University of Medical Sciences, Semnan, Iran
2 Department of Radiopharmacy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
3 Department of Plant and Animal Biology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
4 Department of Pathology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran
5 Department of Pharmacognosy, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran
Abstract
Background: Investigations have shown that estrogen affects wound healing through skin receptors. Astragalus floccosus Boiss. contains estrogen-like isoflavonoids. This study examined the effects of A. floccosus Boiss (A. floccosus) extract on wound healing in vitro and in vivo.
Methods: For the in vitro test, Human Fetal Foreskin Fibroblast 2 (HFFF2) cells treated with the root extract of A. floccosus were followed over a 48-hour period. For the in vivo test, rats were categorized into five groups and received daily applications of a cream containing different concentrations of the extract: 5%, 10%, silver sulfadiazine, the ointment base, and no treatment for a duration of 21 days. The wound size and full-thickness wound healing were measured throughout the inspection, and a histological study was conducted as well. The two-way repeated measures ANOVA test was employed, with a significance level of 0.05 (P≤0.05) being used in all cases.
Results: A. floccosus at 125 ppm significantly improved the closer region and wound healing in HFFF2 cells over all periods of time and showed a significant healing effect during 8-12 h (P=0.0234). Moreover, an increase in fibrosis (compared to the base (P=0.0005), the 10% extract (P<0.0001), silver (P=0.0005), and free (P<0.0001) groups), epithelization (compared to base (P=0.0006), the 10% extract (P=0.0008), silver (P=0.0007), and free (P=0.0006) groups), and proliferation of fibroblasts (compared to the 10% extract and free groups (P=0.0020 and P=0.0024, respectively) were seen in the 5% extract sample on day 10.
Conclusion: Wound healing processes were observed in rats treated with a 5% A. floccosus extract cream. This finding highlights the potential for future studies on wound healing processes using this extract.
Highlights
Fatemeh Akbari (Google Scholar)
Mohammad Azadbakht (Google Scholar)
Keywords
- Gushiken LFS, Beserra FP, Bastos JK, Jackson CJ, Pellizzon CH. Cutaneous Wound Healing: An Update from Physiopathology to Current Therapies. Life (Basel). 2021;11. doi: 10.3390/life11070665. PubMed PMID: 34357037; PubMed Central PMCID: PMCPMC8307436.
- Ahmad MS, Riaz H, Qureshi OS, Maria P. Role of flavonoids as wound healing agent. Phytochemicals: Source of Antioxidants and Role in Disease Prevention. 2018:95-102. DOI: 10.5772/intechopen.79179.
- Dwivedi D, Dwivedi M, Malviya S, Singh V. Evaluation of wound healing, anti-microbial and antioxidant potential of Pongamia pinnata in wistar rats. J Tradit Complement Med. 2017;7:79-85. doi: 10.1016/j.jtcme.2015.12.002. PubMed PMID: 28053891; PubMed Central PMCID: PMCPMC5198820.
- Shahrajabian M, Sun W, Cheng Q. A review of astragalus species as foodstuffs, dietary supplements, a traditional chinese medicine and a part of modern pharmaceutical science. Applied Ecology & Environmental Research. 2019;17:13371-82. doi: 10.15666/aeer/1706_1337113382.
- Wang J, Zhang D, Zhu Y, Mo X, McHugh PC, Tong Q. Astragalus and human mesenchymal stem cells promote wound healing by mediating immunomodulatory effects through paracrine signaling. Regen Med. 2022;17:219-32. doi: 10.2217/rme-2021-0076. PubMed PMID: 35249360.
- El Mohtadi M, Whitehead K, Dempsey-Hibbert N, Belboul A, Ashworth J. Estrogen deficiency - a central paradigm in age-related impaired healing? EXCLI J. 2021;20:99-116. doi: 10.17179/excli2020-3210. PubMed PMID: 33510594; PubMed Central PMCID: PMCPMC7838826.
- Sidhom S, Schneider A, Fang Y, McFadden S, Darcy J, Sathiaseelan R, et al. 17alpha-Estradiol Modulates IGF1 and Hepatic Gene Expression in a Sex-Specific Manner. J Gerontol A Biol Sci Med Sci. 2021;76:778-85. doi: 10.1093/gerona/glaa215. PubMed PMID: 32857104; PubMed Central PMCID: PMCPMC8087270.
- Engeland CG, Sabzehei B, Marucha PT. Sex hormones and mucosal wound healing. Brain Behav Immun. 2009;23:629-35. doi: 10.1016/j.bbi.2008.12.001. PubMed PMID: 19111925; PubMed Central PMCID: PMCPMC2746088.
- Hsieh CY, Santell RC, Haslam SZ, Helferich WG. Estrogenic effects of genistein on the growth of estrogen receptor-positive human breast cancer (MCF-7) cells in vitro and in vivo. Cancer Res. 1998;58:3833-8. PubMed PMID: 9731492.
- Allred CD, Ju YH, Allred KF, Chang J, Helferich WG. Dietary genistin stimulates growth of estrogen-dependent breast cancer tumors similar to that observed with genistein. Carcinogenesis. 2001;22:1667-73. doi: 10.1093/carcin/22.10.1667. PubMed PMID: 11577007.
- Gu Y, Chen X, Fu S, Liu W, Wang Q, Liu KJ, et al. Astragali Radix Isoflavones Synergistically Alleviate Cerebral Ischemia and Reperfusion Injury Via Activating Estrogen Receptor-PI3K-Akt Signaling Pathway. Front Pharmacol. 2021;12:533028. doi: 10.3389/fphar.2021.533028. PubMed PMID: 33692686; PubMed Central PMCID: PMCPMC7937971.
- Mori T, Kitawaki J. Role of estrogen and estrogen-related factors in endometriosis. Immunology of Endometriosis: Elsevier; 2022. p. 105-19. doi: 10.1016/B978-0-12-820661-4.00022-4.
- Podlech D, Zarre S. A taxonomic revision of the genus Astragalus L.(Leguminosae) in the old world (3-volume set). Natural History Museum/Naturhistorisches Museum Wien, Wien. 2013;2:1039-150.
- Massoumi A. Astragalus in the old world. Tehran: Research Institute of Forest and Rangelands Pub; 1998. Persian.
- Akbari F, Azadbakht M, Bagheri A, Vahedi L. In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae). Adv Pharmacol Pharm Sci. 2022;2022:7865015. doi: 10.1155/2022/7865015. PubMed PMID: 35392504; PubMed Central PMCID: PMCPMC8983193.
- Akbari F, Azadbakht M, Bagheri A, Vahedi L. In Silico, In Vitro, and In Vivo Wound Healing Activity of Astragalus microcephalus Willd. Adv Pharmacol Pharm Sci. 2022;2022:2156629. doi: 10.1155/2022/2156629. PubMed PMID: 36238201; PubMed Central PMCID: PMCPMC9553362.
- Grajzer M, Wiatrak B, Gebarowski T, Matkowski A, Grajeta H, Roj E, et al. Chemistry, oxidative stability and bioactivity of oil extracted from Rosa rugosa (Thunb.) seeds by supercritical carbon dioxide. Food Chem. 2021;335:127649. doi: 10.1016/j.foodchem.2020.127649. PubMed PMID: 32738538.
- Felician FF, Yu RH, Li MZ, Li CJ, Chen HQ, Jiang Y, et al. The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum. Chin J Traumatol. 2019;22:12-20. doi: 10.1016/j.cjtee.2018.10.004. PubMed PMID: 30827814; PubMed Central PMCID: PMCPMC6529365.
- Movaffagh J, Fazly Bazzaz BS, Yazdi AT, Sajadi-Tabassi A, Azizzadeh M, Najafi E, et al. Wound Healing and Antimicrobial Effects of Chitosan-hydrogel/Honey Compounds in a Rat Full-thickness Wound Model. Wounds. 2019;31:228-35. PubMed PMID: 31298661.
- Farmoudeh A, Akbari J, Saeedi M, Ghasemi M, Asemi N, Nokhodchi A. Methylene blue-loaded niosome: preparation, physicochemical characterization, and in vivo wound healing assessment. Drug Deliv Transl Res. 2020;10:1428-41. doi: 10.1007/s13346-020-00715-6. PubMed PMID: 32100265; PubMed Central PMCID: PMCPMC7447683.
- Afonso A, Gonçalves J, Luís Â, Gallardo E, Duarte AP. Evaluation of the in vitro wound-healing activity and phytochemical characterization of propolis and honey. Applied Sciences. 2020;10:1845. doi: 10.3390/app10051845.
- Akbari F, Azadbakht M, Bagheri A, Vahedi L. In Vitro and In Vivo Wound Healing Activity of Astragalus floccosus Boiss. (Fabaceae). Adv Pharmacol Pharm Sci. 2022;2022:7865015. doi: 10.1155/2022/7865015. PubMed PMID: 35392504; PubMed Central PMCID: PMCPMC8983193.
- Dorsett-Martin WA. Rat models of skin wound healing: a review. Wound Repair Regen. 2004;12:591-9. doi: 10.1111/j.1067-1927.2004.12601.x. PubMed PMID: 15555049.
- Krischak GD, Augat P, Claes L, Kinzl L, Beck A. The effects of non-steroidal anti-inflammatory drug application on incisional wound healing in rats. J Wound Care. 2007;16:76-8. doi: 10.12968/jowc.2007.16.2.27001. PubMed PMID: 17319622.
- Habibipour S, Oswald TM, Zhang F, Joshi P, Zhou XC, Dorsett-Martin W, et al. Effect of sodium diphenylhydantoin on skin wound healing in rats. Plast Reconstr Surg. 2003;112:1620-7. doi: 10.1097/01.PRS.0000086773.96319.DA. PubMed PMID: 14578793.
- Ali S, Ishteyaque S, Khan F, Singh P, Soni A, Mugale MN. Accelerative Wound-Healing Effect of Aqueous Anthocephalus Cadamba Leaf Extract in a Diabetic Rat Model. Int J Low Extrem Wounds. 2023;22:409-17. doi: 10.1177/15347346211018330. PubMed PMID: 33988470.
- Qian B, Li J, Guo K, Guo N, Zhong A, Yang J, et al. Antioxidant biocompatible composite collagen dressing for diabetic wound healing in rat model. Regen Biomater. 2021;8:rbab003. doi: 10.1093/rb/rbab003. PubMed PMID: 33738117; PubMed Central PMCID: PMCPMC7955720.
- Xu Z, Han S, Gu Z, Wu J. Advances and Impact of Antioxidant Hydrogel in Chronic Wound Healing. Adv Healthc Mater. 2020;9:e1901502. doi: 10.1002/adhm.201901502. PubMed PMID: 31977162.
- Sirotkin AV, Harrath AH. Phytoestrogens and their effects. Eur J Pharmacol. 2014;741:230-6. doi: 10.1016/j.ejphar.2014.07.057. PubMed PMID: 25160742.
- Emmerson E, Campbell L, Ashcroft GS, Hardman MJ. The phytoestrogen genistein promotes wound healing by multiple independent mechanisms. Mol Cell Endocrinol. 2010;321:184-93. doi: 10.1016/j.mce.2010.02.026. PubMed PMID: 20193736.
- Ozay Y, Guzel S, Yumrutas O, Pehlivanoglu B, Erdogdu IH, Yildirim Z, et al. Wound Healing Effect of Kaempferol in Diabetic and Nondiabetic Rats. J Surg Res. 2019;233:284-96. doi: 10.1016/j.jss.2018.08.009. PubMed PMID: 30502261.
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