Iranian Journal of Medical Sciences
Iranian Journal of Medical Sciences

The Effects of Apigenin Isolated from Sungkai Leaves (Peronema canescens J.) on Skin Collagen Density in Male White Mice (Mus musculus) in an Ultraviolet B-Induced Aging Model

Articles InPress

Document Type : Original Article(s)

Authors

Dwisari Dillasamola
Yufri Aldi
Hana Hasibuan

Department of Pharmacology, Faculty of Pharmacy, Universitas Andalas, Padang, Indonesia

10.30476/ijms.2026.109645.4500
Abstract
Background: Skin aging is a multifactorial process driven by intrinsic factors and environmental exposure, particularly ultraviolet radiation, which induces oxidative stress and the degradation of dermal collagen. Given the limited evidence regarding apigenin derived from Peronema canescens, this study aimed to evaluate its protective effects against oxidative stress and collagen degradation in an ultraviolet B (UVB)-induced skin aging model.
Methods: A true experimental study with a post-test-only control group design was conducted from March to August 2025 in Padang, Indonesia. Twenty-five male white mice (Mus musculus) were randomly assigned into five groups (n=5 per group): a negative control, a UVB-exposed positive control, and three UVB-exposed treatment groups receiving apigenin at 25, 50, or 100 mg/Kg body weight (BW) for 30 days. Data were analyzed using SPSS version 27. Normality and homogeneity of variances were assessed using the Shapiro–Wilk and Levene tests, respectively. Malondialdehyde (MDA) levels were analyzed using one-way ANOVA followed by Tukey’s post hoc test, while collagen density scores were analyzed using the Kruskal–Wallis test followed by Dunn’s test with Bonferroni correction.
Results: MDA levels differed significantly among groups (F[4,20]=61.743, P<0.001). The 100 mg/Kg BW group did not differ significantly from the negative control, indicating the restoration of MDA levels to near-normal values. Collagen density also differed significantly among groups (P=0.003), with significant improvement in the 100 mg/Kg BW group compared with the positive control (adjusted P=0.049).
Conclusion: Apigenin derived from P. canescens exhibited dose-dependent antioxidant and dermoprotective effects in a UVB–induced photoaging model.

Highlights

Dwisari Dillasamola (Google Scholar)

Keywords

Apigenin
Malondialdehyde
Collagen
Skin aging
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Iranian Journal of Medical Sciences

Articles InPress, Corrected Proof
Available Online from 20 October 2026


  • Received 11 November 2025
  • Revised 01 May 2026
  • Accepted 15 June 2026

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